| A simple method for the detection of hepatitis A virus in the environmental samples by polymerase chain reaction |
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| 作者姓名： | Yang Feng Xu Xun Zhang Yusheng Chen Rongzhong Zhou Xiulan |
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| 作者单位： | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China;Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| 摘 要： | Hepatitis A virus (HAV) is very persistent in the environment and is especially difficult to detect in the seafoods. In recent years, molecular cloning of the genome of HAV has led to sensitive polymerase chain reaction (PCR) method for the detection of HAV RNA. Here we describe a new and simple procedure to extract RNA from contaminated clams and the PCR method for detection of HAV in environmental samples. The specificity and efficiency of PCR amplification were studied using cDNA and RNA of HAV. Three primer couples gave satisfactory results. Some basic parameters of the PCR were modified to perform a highly specific and sensitive test.
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| 收稿时间： | 1992/4/15 0:00:00 |
| 修稿时间： | 6/3/1992 12:00:00 AM |
A simple method for the detection of hepatitis A virus in the environmental samples by polymerase chain reaction |
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| Yang Feng,Xu Xun,Zhang Yusheng,Chen Rongzhong,Zhou Xiulan.A simple method for the detection of hepatitis A virus in the environmental samples by polymerase chain reaction[J].Acta Oceanologica Sinica,1993,12(2):279-284. |
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| Authors: | Yang Feng Xu Xun Zhang Yusheng Chen Rongzhong and Zhou Xiulan |
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| Institution: | Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China |
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| Abstract: | Hepatitis A virus (HAV) is very persistent in the environment and is especially difficult to detect in the seafoods. In recent years, molecular cloning of the genome of HAV has led to sensitive polymerase chain reaction (PCR) method for the detection of HAV RNA. Here we describe a new and simple procedure to extract RNA from contaminated clams and the PCR method for detection of HAV in environmental samples. The specificity and efficiency of PCR amplification were studied using cDNA and RNA of HAV. Three primer couples gave satisfactory results. Some basic parameters of the PCR were modified to perform a highly specific and sensitive test. |
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