| 重组藻胆蛋白与高等植物类囊体膜之间能量传递的研究 |
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| 引用本文： | 尚孟慧,臧晓南,林娇娇,李瑞,毕莹,徐晓婷.重组藻胆蛋白与高等植物类囊体膜之间能量传递的研究[J].中国海洋大学学报(自然科学版),2021(5). |
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| 作者姓名： | 尚孟慧 臧晓南 林娇娇 李瑞 毕莹 徐晓婷 |
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| 作者单位： | 中国海洋大学海洋生物遗传学与育种教育部重点实验室 |
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| 基金项目： | 国家自然科学基金项目(31872555)资助。 |
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| 摘 要： | 为了探究藻胆蛋白与高等植物类囊体膜之间的光能传递规律,验证重组藻胆蛋白是否可以与高等植物类囊体膜之间的光能传递,本研究分别提取了重组藻红蛋白、重组藻蓝蛋白以及菠菜和韭菜的类囊体进行混合孵育,然后进行了全波长吸收光谱以及荧光光谱检测。结果显示:混合孵育时间为10~20 min有利于重组藻红蛋白和藻蓝蛋白与类囊体膜结合并进行光能传递,最适混合孵育时间随重组藻胆蛋白和类囊体的种类不同而稍有不同;重组藻红蛋白不能与菠菜或韭菜的类囊体膜直接发生光能传递,但是当体系中添加重组藻蓝蛋白的时候就可以;重组藻蓝蛋白可以与类囊体中的叶绿素a直接进行光能传递,使得叶绿素a的荧光发射峰值显著提升,当藻红蛋白和藻蓝蛋白的比例是1∶2时,光能传递效率较高。本研究为藻胆蛋白在构建新型光合系统中的应用提供实验依据。
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| 关 键 词： | 藻红蛋白 藻蓝蛋白 重组表达 类囊体膜 光能传递 |
Study on Energy Transfer Between Recombinant Phycobiliprotein and Thylakoid Membrane |
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| Institution: | (Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao 266003, China) |
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| Abstract: | In order to explore the energy transmission mechanism of phycobiliprotein and thylakoid membrane,and to verify whether the recombinant phycobiliprotein can transmit light energy to the thylakoid membrane of higher plants,recombinant phycocyanin and the thylakoids of leek and spinach were incubated together to test the fullwavelength absorption spectroscopy and fluorescence spectroscopy.The results showed that the mixed incubation time of 10~20 minutes is beneficial to the combination of recombinant phycoerythrin and phycocyanin with thylakoid membrane.The optimal mixing incubation time varies slightly with the types of recombinant phycobiliproteins and thylakoids.Recombinant phycoerythrin cannot directly transmit light energy to the thylakoid membrane of spinach or leek,but when recombinant phycocyanin is added to the system,the energy transformation could occur.The recombinant phycocyanin could transfer light directly to chlorophyll a in thylakoids,and the fluorescence emission peak of chlorophyll a was significantly increased,The light energy transmission efficiency is the highest when the ratio of phycoerythrin and phycocyanin is 1∶2.This study provides experimental evidence for the application of phycobiliprotein in the construction of a new photosynthetic system. |
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| Keywords: | phycoerythrin phycocyanin recombinant expression thylakoid membrane light energy transmission |
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