| 健康与患病大黄鱼消化道微生物结构特征分析 |
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| 引用本文： | 姜燕,于超勇,徐永江,柳学周,郑炜强,陈佳,刘莹,王滨,史宝.健康与患病大黄鱼消化道微生物结构特征分析[J].中国海洋大学学报(自然科学版),2021(5). |
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| 作者姓名： | 姜燕 于超勇 徐永江 柳学周 郑炜强 陈佳 刘莹 王滨 史宝 |
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| 作者单位： | 大黄鱼育种国家重点实验室宁德市富发水产有限公司;中国水产科学研究院黄海水产研究所青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室;山东省海洋生物研究院 |
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| 基金项目： | 大黄鱼育种国家重点实验室开放基金项目(LYC2017RS01);现代农业产业技术体系专项(CARS-47);中央引导地方科技发展专项(2017L3019);福建省科技平台建设项目(2018N2005)资助。 |
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| 摘 要： | 为研究患病大黄鱼(Larimichthys crocea)消化道中可能的病原微生物,采用高通量测序技术详细分析了健康和患病大黄鱼的胃、幽门盲囊和肠道中微生物群的组成信息和分布特征。研究显示,变形菌门、拟杆菌门和厚壁菌门为健康和患病大黄鱼消化道的优势菌门,且其丰度在两类鱼消化道中差异显著。拟杆菌属和乳杆菌属为健康大黄鱼消化道的主要优势菌属;Aliivibrio和弧菌属则为病鱼消化道的主要优势菌属。患病鱼消化道各部位中Aliivibrio的丰度是健康大黄鱼的1702.83~10957.55倍,且差异显著。健康鱼消化道微生物参与的部分代谢通路的基因数目与病鱼样本存在显著差异。大黄鱼消化道菌群和环境菌群相似性分析及主要菌群分布特征显示,饲料中微生物群对消化道菌群的影响相对较大,说明包括饲料在内的环境微生物的卫生质量在健康养殖中的作用非常关键。通过研究推断Aliivibrio和弧菌属为主要病原菌,养殖过程中维持鱼体消化道微生物群的动态平衡对机体健康至关重要,本研究为大黄鱼健康养殖中疾病的定向防控提供了参考。
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| 关 键 词： | 环境菌群 消化道 高通量测序 大黄鱼 微生物群 |
Analysis of Microbiota Structural Characteristic in the Gastrointestinal Tract of Healthy and Diseased Large Yellow Croaker |
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| JIANG Yan,YU Chao-Yong,XU Yong-Jiang,LIU Xue-Zhou,ZHENG Wei-Qiang,CHEN Jia,LIU Ying,WANG Bin,SHI Bao.Analysis of Microbiota Structural Characteristic in the Gastrointestinal Tract of Healthy and Diseased Large Yellow Croaker[J].Periodical of Ocean University of China,2021(5). |
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| Authors: | JIANG Yan YU Chao-Yong XU Yong-Jiang LIU Xue-Zhou ZHENG Wei-Qiang CHEN Jia LIU Ying WANG Bin SHI Bao |
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| Institution: | (State Key Laboratory of Large Yellow Croaker Breeding, Ningde Fufa Fisheries Co. Ltd., Ningde 352103, China;Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Marine Biology Institute of Shandong Province, Qingdao 266104, China) |
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| Abstract: | The objective of this program was to seek main microbiota in gastrointestinal tract of disea-sed large yellow croaker(Larimichthys crocea).We carefully analyzed microbiota composition and distribution in stomach,pyloric caecum and gut of large yellow croaker under different healthy conditions by high-throughput sequencing.Results showed that the microbiota diversity of pyloric caecum and gut was significantly different from that of stomach of healthy fish.However,these differences changed in diseased fish.The structural characteristics of microbiota in pyloric caecum and gut were more similar in these two fish.Proteobacteria,Bacteroidetes and Firmicutes were the dominant phyla in these two kinds of large yellow croaker gastrointestinal tract although significant differences existed in each position of gastrointestinal tract.Bacteroides and Lactobacillus were the dominant genera in healthy fish gastrointestinal tract while Aliivibrio and Vibrio in diseased fish.Abundance of Aliivibrio in diseased fish gastrointestinal tract is 1702.83~10957.55 times of that in healthy fish gastrointestinal tract,and the difference is significant.There were significant differences between numbers of genes involved in part of the metabolic pathway in gastrointestinal tract of healthy fish and that in diseased fish.Compared with the water,similarity analysis of gastrointestinal tract microbiota and environmental microbiota and distribution characteristics of the main microbiota showed that the microbiota in feed had a relatively greater impact on the gastrointestinal microbiota,indicating that the quality of environmental microbiota including feed was crucial in healthy breeding.Through this study,it was concluded that Aliivibrio and Vibrio were the main pathogenic bacteria for large yellow croaker,and maintaining the dynamic balance of gastrointestinal tract microbiota of fish during farming is important for the health of the body,which will provide a reference for the directional prevention and control of diseases in the healthy culture of large yellow croaker. |
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| Keywords: | environmental microbiota gastrointestinal tract high throughput sequencing Larimichthys crocea microbiota |
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