| POLYCLONAL ANTIBODIES TO LIGHT-HARVESTING CHL-PROTEIN OF PSII (LHC II) IN MARINE GREEN ALGAE BRYOPSIS CORTICULANS |
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| 作者姓名： | 仵小南 周百成 曾呈奎 |
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| 作者单位： | E.M.B.L.，Institute of Oceanology，Academia Sinica，Qingdao 266071，E.M.B.L.，Institute of Oceanology，Academia Sinica，Qingdao 266071，E.M.B.L.，Institute of Oceanology，Academia Sinica，Qingdao 266071 |
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| 基金项目： | Contribution No 1717 from the Institute of Oceanology, Academia Sinica,This subject was supported by the National Science Foundation of China. |
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| 摘 要： | Polyc . lonal antibodies raised against LHC II isolated from SDS-solubilized Bryopsis corticulans thylakiod membranes by SDS-PAGE, were characterised by double immunodifiusion, Rocket immunoelectrophoresis and antigen-antibody crossed immunoelectro - phoresis assays showed the antibodies had strong cross-reaction with all B , corticulans LHC II components (even with those which were incubated in boiling water)and showed immunological cross-reactivity with LHC II polypeptides of spinach and the marine green alga Codium fragile. The results suggested that LHC II of different species had similar antigenic determinants and also conservation of amino acid sequences of the polypeptides during evolution, and that the antibodies could cross react with apoproteins of D2 proteins (which contain P680) from B. corticulans, spinach and C. fragile, but not with apoproteins of P 700 Chl-proteins. Our results indicated some similarities in primary structure between LHC II of different species, and between LHC II and D2
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POLYCLONAL ANTIBODIES TO LIGHT-HARVESTING CHL-PROTEIN OF PSII (LHC II) IN MARINE GREEN ALGAE BRYOPSIS CORTICULANS |
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| WU Xiaonan ZHOU Baicheng and C .K .TSENG.POLYCLONAL ANTIBODIES TO LIGHT-HARVESTING CHL-PROTEIN OF PSII (LHC II) IN MARINE GREEN ALGAE BRYOPSIS CORTICULANS[J].Chinese Journal of Oceanology and Limnology,1992(2). |
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| Authors: | WU Xiaonan ZHOU Baicheng and C K TSENG |
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| Abstract: | Polyc . lonal antibodies raised against LHC II isolated from SDS-solubilized Bryopsis corticulans thylakiod membranes by SDS-PAGE, were characterised by double immunodifiusion, Rocket immunoelectrophoresis and antigen-antibody crossed immunoelectro - phoresis assays showed the antibodies had strong cross-reaction with all B , corticulans LHC II components (even with those which were incubated in boiling water)and showed immunological cross-reactivity with LHC II polypeptides of spinach and the marine green alga Codium fragile. The results suggested that LHC II of different species had similar antigenic determinants and also conservation of amino acid sequences of the polypeptides during evolution, and that the antibodies could cross react with apoproteins of D2 proteins (which contain P680) from B. corticulans, spinach and C. fragile, but not with apoproteins of P 700 Chl-proteins. Our results indicated some similarities in primary structure between LHC II of different species, and between LHC II and D2 proteins of marine green algae and spin -ach . Our finding that D2 and P700 Chl-proteins are not immunologically related suggested that P700 Chl-proteins and D2 proteins pass through independent evolutionary pathways . |
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| Keywords: | LHC antibodies marine algae evolution |
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