| 塞舌尔角毛藻的形态学再描述和分子系统学分析 |
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| 引用本文： | 牛彪彪,翟梦怡,李扬.塞舌尔角毛藻的形态学再描述和分子系统学分析[J].热带海洋学报,2021,40(4):44-49. |
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| 作者姓名： | 牛彪彪 翟梦怡 李扬 |
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| 作者单位： | 华南师范大学生命科学学院, 广东省水产健康安全养殖重点实验室, 广东 广州 510631 |
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| 基金项目： | 科技基础资源调查专项(2018FY100200) |
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| 摘 要： | 塞舌尔角毛藻(Chaetoceros seychellarus)属于硅藻门角毛藻属有色角毛藻亚属, 为全球罕见种。以往有关塞舌尔角毛藻的研究报道多限于光学显微镜观察, 对其形态学认知仍不完整, 系统学研究也尚属空白。本文从我国台湾海峡和海南岛三亚海域采集了塞舌尔角毛藻, 并在实验室建立了单克隆培养株系, 利用光镜、扫描电镜对其形态学特征进行了较为详尽的观察, 基于核糖体大亚基(large subunit ribosomal DNA)D1~D3区序列, 分析了其系统学信息。塞舌尔角毛藻的典型特征是链状群体直, 窗孔呈矩形, 壳面具有两个唇形突。分子系统树显示, 塞舌尔角毛藻与丹麦角毛藻构成姐妹支, 依据形态学建立的北方组呈现多系起源, 后续有待加以修订。
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| 关 键 词： | 塞舌尔角毛藻 形态学 分子系统 北方组 |
| 收稿时间： | 2020-06-19 |
| 修稿时间： | 2020-08-08 |
Morphological and Phylogenetic Studies on Chaetoceros seychellarus |
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| NIU Biaobiao,ZHAI Mengyi,LI Yang.Morphological and Phylogenetic Studies on Chaetoceros seychellarus[J].Journal of Tropical Oceanography,2021,40(4):44-49. |
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| Authors: | NIU Biaobiao ZHAI Mengyi LI Yang |
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| Institution: | Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, China |
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| Abstract: | Chaetoceros seychellarus, which belongs to subgenus Phaeoceros of genus Chaetoceros in Bacillariophyta, is a rare species in the world. Present knowledge on the morphology is incomplete, because studies are mainly conducted under LM (light microscope) and lack ultrastructural characteristics under EM (electron microscope). Its systematic information is still unknown. In this study, monoclonal strains of C. seychellarus were established from coastal waters in the southeast China. The morphology was observed using LM and SEM (scanning electron microscope). D1-D3 regions of the large subunit ribosomal DNA (LSU) were amplified to construct the phylogenetic relationship. The key features were straight chains, rectangular apertures and two rimoportulae on each valve that distributed symmetrically along the transapical axis. The phylogenetic tree showed C. seychellarus was a sister to C. danicus. On LSU tree, the section Borealia established based on morphology solely was paraphyletic, which needs revision in further. |
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| Keywords: | Chaetoceros seychellarus morphology phylogeny section Borealia |
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