| 运用RAPD技术对笛鲷属3种鱼的群体遗传学分析 |
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| 引用本文： | 刘丽,刘楚吾,许强.运用RAPD技术对笛鲷属3种鱼的群体遗传学分析[J].广东海洋大学学报,2003,23(3):16-21. |
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| 作者姓名： | 刘丽 刘楚吾 许强 |
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| 作者单位： | 湛江海洋大学海洋生物研究所,广东,湛江,524025 |
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| 基金项目： | 广东省重大科技兴海项目(A200099A01)，湛江市科技攻关项目(2000-17) |
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| 摘 要： | 应用RAPD技术,从9个系列180种随机引物中筛选出16种,对笛(鱼周)属的画眉笛(鱼周)(Lutja-nus vitta)、金焰笛(鱼周)(Lutjanus fulviflamma)、金带笛(鱼周)(Lutjanus vaigiensis)进行种群内及种群间遗传学分析,并利用UPGMA确定了它们之间的亲缘关系。结果表明,三种鱼的种内遗传多样性指数(H)分别为,画眉笛(鱼周)0.1949,金带笛(鱼周)0.1107,金焰笛(鱼周)0.1673;三者的多态位点比例(P)分别为69.4％、47.8％和59.0％;金带笛(鱼周)具有较低的遗传多样性,且全带笛(鱼周)与金焰笛(鱼周)有较近的亲缘关系。三种鱼共检出10个可作为种的特异性鉴定的条带,可用于种质鉴定。
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| 关 键 词： | 画眉笛鲷 金焰笛鲷 金带笛鲷 遗传多样性 种质鉴定 RAPD |
| 文章编号： | 1007-7995(2003)03-0016-06 |
| 修稿时间： | 2003年2月23日 |
Population Genetic Analysis on Three Species of Snappers by Random Amplified Polymorphic DNA (RAPD) |
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| LIU,LIU Chu-wu,XU Qiang.Population Genetic Analysis on Three Species of Snappers by Random Amplified Polymorphic DNA (RAPD)[J].Journal of Zhanjiang Ocean University,2003,23(3):16-21. |
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| Authors: | LIU LIU Chu-wu XU Qiang |
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| Abstract: | 16 random primers are used in the population genetic analysis on three species of snappers:Lutjanus vitta, Lutjanus vaigiensis and Lutjanus fulviflamma by RAPD technique. The relationship among three species are analyzed by using UPGMA . The genetic diversity indexes of intra-species (H) are 0. 194 9, 0. 110 7 and 0. 167 3 respectively. The polymorphic loci ratio(P) of L. vitta, L. vaigiensis and L. fa.lviflam.ma are 69.4% , 47.8% and 59.0% respectively. A closest genetic relationship exists between L. vaigiensis and L. fulviflamma. 10 species-specific markers are found and they can be used as molecular genetic markers to identify the three species of snapper. |
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| Keywords: | Lutjanus vitta Lutjanus vaigiensis Lutjanus fulviflamma genetic diversity species-specific markers RAPD |
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