| Analysis of IL-2-1ike factor in lymphocyte culture supernatant of olive flounder, Paralichthys oliveaceus |
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| 基金项目： | This work was supported by National “973” Project G1999012003,G19999012006. |
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| 摘 要： |
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| 关 键 词： | 淋巴细胞 橄榄叶 免疫组织 鱼 ELISA |
| 收稿时间： | 2003-10-08 |
| 修稿时间： | 2004-07-02 |
Analysis of IL-2-like factor in lymphocyte culture supernatant of olive flounder,Paralichthys oliveaceus |
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| Authors: | Wu Riqin Zhang Peijun Li Jun Xu Yongli |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Institute of Postgraduate, Chinese Academy of Sciences, 100039 Beijing, China |
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| Abstract: | To study immune mechanism of fish lymphocyte we performed a proliferation assay and ELISA using monoclonal antibody against
human IL-2. The result showed that an interleukin-2 (IL-2)-like factor was detected in the supernatant of plant haemoglutinin
(PHA)-stimulated lymphocyte culture from peripheral blood, spleen and head kidney of olive flounder,Paralichthys olivaceus. The quantities of IL-2-like factor in the supernatant from different lymphoid tissues were quite different. The IL-2 like
factor in the supernatant from cultured head kidney lymphocytes was much higher than those of peripheral blood lymphocytes
and spleen lymphocytes (P<0.01). The IL-2 activity was found in either mouse thymocyte proliferation assay or flounder head kidney lymphocyte proliferation
assay and shown to have obvious enhancing effect on proliferation of the above two types of cell. The recombinant human IL-2,
(rhIL-2) was able to stimulate flounder thymocyte proliferation and used to detect the IL-2 receptor (IL-2R) on the surface
of flounder lymphocyte. The cross-reaction between the lymphocytes of flounder peripheral blood and CD25(IL-2R) was detected
with flow cytometry and shown that the percentage of CD25-positive cell in peripheral blood was 7.74±0.67%.
This work was supported by National “973” Project G1999012003, G19999012006. |
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| Keywords: | Interleukin-2 IL-2 receptor olive flounder lymphocyte |
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