| Occurrence of Two Distinct Types of Tissue Inhibitors of Metallo-proteinases-2 in Fugu rubripes |
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| 作者姓名： | Yoshihiro Yokoyama Hiroshi Tsukamoto Tohru Suzuki Shohshi Mizuta Reiji Yoshinaka |
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| 作者单位： | [1]Faculty of Biotechnology, Fukui Prefectural University, Japan [2]Graduate School of Agricultural Science, Tohoku University, Japan |
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| 基金项目： | 日本教育、科学、文化部资助项目 |
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| 摘 要： | In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 （TIMP-2） from Japanese puffer fish Fugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAs are composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteine residues, which might form six disulfide bonds as in other animals＇ TIMP-2s. Reverse-transcribed polymerase chain reaction analysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expression patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues.
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| 关 键 词： | 河豚 蛋白酶 抑制剂 稳定性 多样性 |
| 文章编号： | 1672-5182（2005）03-214-05 |
| 收稿时间： | 2005-01-21 |
| 修稿时间： | 2005-05-30 |
Occurrence of Two Distinct Types of Tissue Inhibitors of Metallo-proteinases-2 in Fugu rubripes |
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| Yoshihiro Yokoyama Hiroshi Tsukamoto Tohru Suzuki Shohshi Mizuta Reiji Yoshinaka.Occurrence of Two Distinct Types of Tissue Inhibitors of Metallo-proteinases-2 in Fugu rubripes[J].Journal of Ocean University of China,2005,4(3):214-218. |
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| Authors: | Yoshihiro Yokoyama Hiroshi TSUKAMOTO Tohru Suzuki Shohshi Mizuta Reiji Yoshinaka |
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| Abstract: | In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fishFugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAsare composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteineresidues, which might form six disulfide bonds as in other animals' TIMP-2s. Reverse-transcribed polymerase chain reactionanalysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expres-sion patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues. |
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| Keywords: | Fugu rubripes TIMP-2 tissue inhibitor of metalloproteinase |
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