| Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri |
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| 作者姓名： | 张洋 张晓军 张洪斌 李富花 相建海 |
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| 作者单位： | [1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Graduate School, Chinese Academy of Sciences, Beijing 100049, China [3]Department of Soil ＆ Crop Sciences, Texas A＆M University, College Station, 77843, USA |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划) |
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| 摘 要： | Two Large-insert genomic bacterial artificial chromosome （BAC） libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.
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| 关 键 词： | BAC库 扇贝 基因组 海洋开发 |
| 收稿时间： | 15 October 2007 |
Construction of bacterial artificial chromosome libraries for Zhikong Scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis> |
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| Yang?Zhang,Xiaojun?Zhang,Chantel?F.?Scheuring,Hongbin?Zhang,Fuhua?Li,Jianhai?Xiang.Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri[J].Chinese Journal of Oceanology and Limnology,2008,26(2):215-218. |
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| Authors: | Yang Zhang Xiaojun Zhang Chantel F Scheuring Hongbin Zhang Fuhua Li Jianhai Xiang |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;(2) Graduate School, Chinese Academy of Sciences, Beijing, 100049, China;(3) Department of Soil & Crop Sciences, Texas A&M University, College Station, 77843, USA |
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| Abstract: | Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research.High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I,respectively.The BamH I library consisted of 53760 clones while the Mbo I library consisted of 7680 clones.Approximately 96% of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb,providing a coverage of 5.3 haploid genome equivalents.Similarly,the Mbo I library with an average insert of 145 kb and no insert-empty clones,thus providing a genome coverage of 1.1 haploid genome equivalents. |
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| Keywords: | BAC library Zhikong scallop Chlamys farreri genome |
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