Antiviral active peptide from oyster |
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| Authors: | Mingyong Zeng Wenxuan Cui Yuanhui Zhao Zunying Liu Shiyuan Dong Yao Guo |
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| Institution: | College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China |
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| Abstract: | An active peptide against herpes virus was isolated from the enzymic hydrolysate of oyster (Crassostrea gigas) and purified with the definite direction hydrolysis technique in the order of alcalase and bromelin. The hydrolysate was fractioned into four ranges of molecular weight (>10 kDa, 10–5 kDa, 5–1 kDa and <1 kDa) using ultrafiltration membranes and dialysis. The fraction of 10–5 kDa was purified using consecutive chromatographic methods including DEAE Sephadex A-25 column, Sephadex G-25 column, and high performance liquid chromatogram (HPLC) by activity-guided isolation. The antiviral effect of the obtained peptide on herpetic virus was investigated in Vero cells by observing cytopathic effect (CPE). The result shows that the peptide has high inhibitory activity on herpetic virus. |
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| Keywords: | Crassostrea gigas antiviral activity herpetic-virus active peptide |
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