Effects of different combinations of cryoprotectants on spermatozoon vitality of Chinese scallop,Chlamys farreri,during low temperature equilibrium |
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| Authors: | Xue Qin-zhao Xiang Jian-hai |
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| Institution: | (1) Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao |
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| Abstract: | The effects of membrane penetrating anti—freezing agents (MPAAs), DMSO (dimethyl sulfoxide), glycerol, EG (ethylene glycol)
and methanol in combination with different cryoprotective additives such as carbohydrates, macromoleules and inorganic compounds
on the spermatozoon vitality of Chinese scallop,Chlamys farreri, during 1 h 0 °C equilibrium were investigated. When only MPAAs existed, the detrimental effects of different MPAAs ranked
in the following order: DMSO<methanol<EG<glycerol. When carbohydrates were added into MPAAs solution, 5% glucose caused larger
decrease of spermatozoon vitality than 2.4% lactose. 5% glucose or 2.4% lactose in 7.5% glycerol caused complete damage. 10%
yolk was best in maintaining the spermatozoon vitality except when used in combination with 10% methanol. 10% milk significantly
decreased spermatozoon vitality in EG and methanol and enhanced its vitality in glycerol, but did not significantly influence
it in DMSO. Glycine MPAAs ranked in the following order. 10% yolk <10% yolk +2.4% lactose <2.4% lactose <2.0% citrate <10%
milk <10% milk +2.0% citrate <5% glucose <80mmol/L glycine +55 mmol/L NaHCO3.
Contribution No. 2434 from the Institute of Oceanology, Chinese Academy of Sciences. |
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| Keywords: | spermatozoon vitality Chlamys farreri cryoprotectants equilibrium |
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