| Enzymatic properties of UFE, a novel marine fibrinolytic enzyme |
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| 作者姓名： | WANG Dianliang LIU Wanshun HAN Baoqin |
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| 作者单位： | WANG Dianliang1，LIU Wanshun1，HAN Baoqin11. College of Marine Life Sciences，Ocean University of China，Qingdao 266003，China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | A novel potent protease, Urechis unicinctus fibrinolytic enzyme (UFE), was firstly discovered. The enzymatic properties of UFE were further investigated. As a low molecular mass protein, UFE appeared to be very stable to heat and pH. When temperature was below 50 ℃, the remnant enzyme activity remained almost unchanged, but when temperature was raised to 60 ℃, the remnant enzyme activity began to decrease rapidly. UFE was quite stable in the range of pH value from 3 to 12, especially in slightly alkaline pH value. Mn2 , Cu2 and Fe2 ions were activators of UFE, while Fe3 and Ag ions were inhibitors of UFE. Fe2 ion along with Fe3 ion might regulate UFE activity in vivo. The optimum pH and temperature of UFE were about 8 and 50 ℃, respectively. Other characteristics of this enzyme were also studied. Systematic research results are significant when UFE is applied for medical and industrial purposes.
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| 关 键 词： | 海洋动物药 单环刺螠 海肠子 纤维蛋白溶解酶 UFE 酶学性质 |
| 收稿时间： | 2006-04-03 |
| 修稿时间： | 2006-08-28 |
Enzymatic properties of UFE, a novel marine fibrinolytic enzyme |
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| WANG Dianliang,LIU Wanshun,HAN Baoqin.Enzymatic properties of UFE, a novel marine fibrinolytic enzyme[J].Acta Oceanologica Sinica,2007,26(2):84-93. |
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| Authors: | WANG Dianliang LIU Wanshun and HAN Baoqin |
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| Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China |
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| Abstract: | A novel potent protease, Urechis unicinctus fibrinolytic enzyme (UFE), was firstly discovered. The enzymatic properties of UFE were further investigated.As a low molecular mass protein,UFE appeared to be very stable to heat and pH.When temperature was below 50 °C ,the remnant enzyme activity remained almost unchanged, but when temperature was raised to 60 °C ,the remnant enzyme activity began to decrease rapidly. UFE was quite stable in the range of pH value from 3 to 12,especially in slightly alkaline pH value.Mn2+ ,Cu2+ and Fe2+ ions were activators of UFE, while Fe3+ and Ag+ ions were inhibitors of UFE.Fe2+ ion along with Fe3+ ion might regulate UFE activity in vivo. The optimum pH and temperature of UFE were about 8 and 50 °C ,respectively. Other characteristics of this enzyme were also studied. Systematic research results are significant when UFE is applied for medical and industrial purposes. |
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| Keywords: | marine animal fibrinolytic enzyme protease enzyme properties |
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