Comparative analysis of methods for concentrating venom from jellyfish Rhopilema esculentum Kishinouye |
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| Authors: | Li Cuiping Yu Huahua Feng Jinhua Chen Xiaolin Li Pengcheng |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China;(2) Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China |
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| Abstract: | In this study, several methods were compared for the efficiency to concentrate venom from the tentacles of jellyfish Rhopilema esculentum Kishinouye. The results show that the methods using either freezing-dry or gel absorption to remove water to concentrate
venom are not applicable due to the low concentration of the compounds dissolved. Although the recovery efficiency and the
total venom obtained using the dialysis dehydration method are high, some proteins can be lost during the concentrating process.
Comparing to the lyophilization method, ultrafiltration is a simple way to concentrate the compounds at high percentage but
the hemolytic activities of the proteins obtained by ultrafiltration appear to be lower. Our results suggest that overall
lyophilization is the best and recommended method to concentrate venom from the tentacles of jellyfish. It shows not only
the high recovery efficiency for the venoms but high hemolytic activities as well.
Supported by the Award Foundation of Scientific Research for Excellent Young and Middle-age Scientist of Shandong Province
(No. 2006BS07003) and the Knowledge Innovation Project of Chinese Academy of Sciences (KZCX2-YW-R-104) |
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| Keywords: | Keyword" target="_blank">Keyword concentration hemolytic activity Rhopilema esculentum Kishinouye venom |
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