| Co-monitoring bacterial and dinoflagellates communities by denaturing gradient gel electrophoresis(DGGE) and SSU rRNA sequencing during a dinoflagellates bloom |
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| 作者姓名： | KAN Jinjun CHEN Feng |
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| 作者单位： | CenterofMarineBiotechnology,UniversityofMarylandBiotechnologyInstitute,Baltimore,MD21202 |
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| 摘 要： | Dinoflagellates are unicellular eukaryotic protists that dominate in all coastal waters, and are also present in oceanic waters. Despite the central importance of dinoflagellates in global primary production, the relationship between dinoflagellates and bacteria are still poorly understood. In order to understand the ecological interaction between bacterial and dinoflageUates communities, denaturing gradient gel electrophoresis (DGGE) and SSU rRNA sequencing were applied to monitoring the population dynamics of bacteria and dinoflagellates from the onset to disappearance of a dinoflagellates bloom occurred in Baltimore Inner Harbor, from April 15 to 24, 2002. Although Prorocentrum minimum was the major bloom forming species under the light microscopy, DGGE method with dinoflagellate specific primers demonstrated that Prorocentrum micans, Gymnodinium galatheanum and Gyrodinium uncatenum were also present during the bloom. Population shifts among the minor dinoflagellate groups were observed. DGGE of PCR-amplified 16S rRNA gene fragments indicated that cyanobacteria, α, β, γ-proteobacteria, FlavobacteriumBacteroides-Cytophaga (FBC), and Planctomcetes were the major components of bacterial assemblages during the bloom. DGGE analysis showed that Cytophagales and α-proteobacteria played important roles at different stages of dinoflagellates bloom. DGGE can be used as a rapid tool to simultaneously monitor population dynamics of both bacterial and dinoflagellates communities in aquatic environments, which is demonstrated here.
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| 关 键 词： | 钴 细菌跟踪 腰鞭毛虫群落 海洋生态系统 变性凝胶体电泳 |
| 收稿时间： | 2004/3/25 0:00:00 |
| 修稿时间： | 6/9/2004 12:00:00 AM |
Co-monitoring bacterial and dinoflagellates communities by denaturing gradient gel electrophoresis(DGGE) and SSU rRNA sequencing during a dinoflagellates bloom |
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| KAN Jinjun,CHEN Feng.Co-monitoring bacterial and dinoflagellates communities by denaturing gradient gel electrophoresis(DGGE) and SSU rRNA sequencing during a dinoflagellates bloom[J].Acta Oceanologica Sinica,2004,23(3):483-492. |
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| Authors: | KAN Jinjun and CHEN Feng |
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| Institution: | Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD 21202 |
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| Abstract: | Dinoflagellates are unicellular eukaryotic protists that dominate in all coastal waters,and are also present in oceanic waters.Despite the central importance of dinoflagellates in global primary production,the relationship between dinoflagellates and bacteria are still poorly understood.In order to understand the ecological interaction between bacterial and dinoflagellates communities,denaturing gradient gel electrophoresis(DGGE) and SSU rRNA sequencing were applied to monitoring the population dynamics of bacteria and dinoflagellates from the onset to disappearance of a dinoflagellates bloom occurred in Baltimore Inner Harbor,from April 15 to 24,2002.Although Prorocentrum minimum was the major bloom forming species under the light microscopy,DGGE method with dinoflagellate specific primers demonstrated that Prorocentrum micans,Gymnodinium galatheanum and Gyrodinium uncatenum were also present during the bloom.Population shifts among the minor dinoflagellate groups were observed.DGGE of PCR-amplified 16S rRNA gene fragments indicated that cyanobacteria,α,β,γ proteobacteria,Flavobacterium-Bacteroides-Cytophaga(FBC),and Planctomcetes were the major components of bacterial assemblages during the bloom.DGGE analysis showed that Cytophagales and α-proteobacteria played important roles at different stages of dinoflagellates bloom.DGGE can be used as a rapid tool to simultaneously monitor population dynamics of both bacterial and dinoflagellates communities in aquatic environments,which is demonstrated here. |
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| Keywords: | bacteria dinoflagellates DGOE SSU rDNA sequencing |
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