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Pfiesteria shumwayae (Pfiesteriaceae) in New Zealand
Authors:Lesley L Rhodes  Joann M Burkholder  Howard B Glasgow  Parke A Rublee  Coy Allen  Janet E Adamson
Institution:1. Cawthron Institute , Private Bag 2, Nelson, New Zealand;2. Center for Applied Aquatic Ecology , North Carolina State University , 620 Hutton St., Suite 104, Raleigh, NC, 27606, United States;3. University of North Carolina at Greensboro , P.O. Box 26 174, Greensboro, NC, 27412, United States
Abstract:Pfiesteria shumwayae Steidinger et Burkholder is now known to be present in New Zealand and occurs in estuaries around the country. The presence of Pfiesteria was initially determined by a polymerase chain reaction (PCR)‐based detection assay, using oligonucleotide primers targeted at ribosomal DNA extracted from estuarine water and sediments. Presence was confirmed by isolation from fresh sediments in the presence offish (Oreochromis mossambicus), followed by identification by scanning electron microscopy. The New Zealand isolates of P. shumwayae were ichthyotoxic in bioassays, but there is no historic evidence offish kills in New Zealand associated with the dinoflagellate.
Keywords:Pfiesteria  dinoflagellate  estuarine  fish kills  molecular probes
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