Modification of the polyethylene glycol 6000 precipitation method for recovering human and indicator viruses from oysters and mussels |
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| Authors: | Gillian D Lewis Angela Hough David H Green John E Hay Lynnette R Ferguson |
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| Institution: | 1. School of Environmental and Marine Sciences , University of Auckland , Private Bag 92019, Auckland, New Zealand;2. Cancer Research Laboratory , The University of Auckland Medical School , Private Bag 92019, Auckland, New Zealand |
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| Abstract: | Human viruses are a common contaminant of shellfish affected by human sewage wastes. They are difficult to detect as they are not easily separated from shellfish tissue. This paper describes a modification of the polyethylene glycol (PEG) precipitation technique for recovery of enteroviruses and F‐specific bacteriophages from the Pacific oyster (Crassostrea gigas) and the green‐lipped mussel (Perna canaliculus). Modifications adopted were the use of only the digestive gland tissue for virus extraction, resuspension of the primary PEG pellet in 4 volumes of eluent, and the introduction of a secondary PEG precipitation to reconcentrate the virus containing extract. The recovery rate of the virus extraction process was not affected by introduction of the secondary concentration step (overall recovery remained at 60–70% of the virus input). The advantages of reduction of tissue residue in the extract, smaller final volume, and the ability to process 2–3 times the number of individual shellfish for the same effort, improve the practicality of the method. |
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| Keywords: | human virus virus enterovirus F RNA bacteriophage shellfish mussel oyster |
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