Quantitation of metallothionein mRNA from the New Zealand common bully (Gobiomorphus cotidianus) and its implications for biomonitoring |
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| Authors: | Andrew D Laurie |
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| Institution: | 1. Landcare Research—Manaaki Whenua , P.O. Box 69, Lincoln, 8152, New Zealand;2. Canterbury Health Laboratories , P.O. Box 151, Christchurch, New Zealand E-mail: Andrew.Laurie@cdhb.govt.nz |
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| Abstract: | Metallothionein (MT) is a protein found in most eukaryotes where its primary role is the regulation of homeostasis of the essential metals copper and zinc. MTs have an additional protective role through their binding of toxic metals such as cadmium and mercury. Increased expression of MT in response to harmful levels of these metals has been demonstrated for several aquatic species. In this study, the expression of MT in the liver tissue of the New Zealand common bully Gobiomorphus cotidianus was measured by quantifying MT mRNA. A statistically significant increase in MT expression was observed in fish exposed to copper for 48 h, but not those exposed to zinc. Field sampling of common bullies showed a wide variation in individual hepatic MT mRNA levels which did not correlate with fish age, sex, or sampling location. A comparison of two populations of common bullies from a polluted and a control site showed a two‐fold higher mean MT mRNA level in fish from the polluted site. Implications for using MT expression in the common bully as a bio‐monitoring tool are discussed. |
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| Keywords: | metallothionein Taqman‐PCR mRNA Gobiomorphus cotidianus biomarker sentinel species |
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