| Cr6+胁迫对水花生(Alternanthera philoxeroides)愈伤组织多胺代谢的影响 |
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| 引用本文： | 田秀丽,施国新,徐君,贾荣,乔绪强,徐小颖,杨海燕,王娟.Cr6+胁迫对水花生(Alternanthera philoxeroides)愈伤组织多胺代谢的影响[J].湖泊科学,2011,23(6):955-960. |
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| 作者姓名： | 田秀丽 施国新 徐君 贾荣 乔绪强 徐小颖 杨海燕 王娟 |
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| 作者单位： | 南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,南京210046 |
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| 摘 要： | 利用植物组织培养技术培养水花生愈伤组织并以此为实验材料,研究了不同Cr6+浓度(0、0.1、0.3、0.6、0.9 mmol/L)对水花生愈伤组织中腐胺(putrescine,Put)、亚精胺(spermidine,Spd)、精胺(spermine,Spm)、脯氨酸(Pro)含量及精氨酸脱羧酶(ADC)、鸟氨酸脱羧酶(...
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| 关 键 词： | 愈伤组织 水花生 Cr6+胁迫 多胺 代谢 |
| 收稿时间： | 2010/11/22 0:00:00 |
| 修稿时间： | 3/2/2011 12:00:00 AM |
Effect of chrome stress on polyamine metabolism of callus of Alternanthera philoxeroides |
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| TIAN Xiuli,SHI Guoxin,XU Jun,JIA Rong,QIAO Xuqiang,XU Xiaoying,YANG Haiyan and WANG Juan.Effect of chrome stress on polyamine metabolism of callus of Alternanthera philoxeroides[J].Journal of Lake Science,2011,23(6):955-960. |
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| Authors: | TIAN Xiuli SHI Guoxin XU Jun JIA Rong QIAO Xuqiang XU Xiaoying YANG Haiyan and WANG Juan |
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| Institution: | Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China,Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China,Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China,Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China,Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China,Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China,Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China and Jiangsu Key Laboratory of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China |
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| Abstract: | Callus of Alternanthera philoxeroides, as experimental material, was cultured by tissue culture technique.In this study, the calluses were subjected to different concentrations of Cr6+ (0, 0.1, 0.3, 0.6 and 0.9 mmol/L) and the impacts on contents of putrescine (Put), spermidine (Spd), spermine (Spm)and proline(Pro), activities of arginine decarboxylase (ADC), orni-thine decarboxylase(ODC), polyamine oxidase(PAO) and Diamine Oxidase(DAO) were investigated.Results indicated that, with the increasing of Cr6+ concentration, (1) the contents of three free PAs increased at first and then decreased, and reached their peak values at 0.6 mmol/L Cr6+ treatments;(2) the content of PS-conjugated Put reached its peak value at 0.1 mmol/L Cr6+ treatment and then decreased slowly, while the contents of PS-conjugated spermidine (Spd) and spermine (Spm) increased significantly;(3) the peak values of PIS-conjugated Put and Spd were present at 0.6 mmol/L Cr6+ treatment;meantime the peak value of PIS-conjugated Spm was present at 0.1 mmol/L Cr6+ treatment;(4)the activities of ADC and ODC increased gradually, while PAO and DAO activities decreased sharply after a transient increase;(5) the level of Pro increased at 0.1-0.3 mmol/L Cr6+ treatments and decreased at 0.6-0.9 mmol/L Cr6+ treatments.These results suggested that Cr6+ stress disturbed metabolic balance of polyamines and increased the level of PAs.Obviously, the changes of PAs levels might be essential to Cr6+ adaption of cells. |
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| Keywords: | Callus Alternanthera philoxeroides Cr6+ stress polyamines metabolism |
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