The Biscayne Aquifer (Florida, USA) is a coastal, shallow, unconfined, and heterogeneous aquifer with high water tables, composed of less-permeable sand to highly permeable karstic limestone. These properties make the Biscayne Aquifer one of the world’s most productive groundwater resources. The aquifer’s high yield and non-Darcian flow cause challenges for estimating aquifer parameters, which are essential for understanding groundwater processes and managing and protecting the groundwater resources. Water-table fluctuations in the Biscayne Aquifer are associated with astronomical tidal forces and gate operations on canal water-control structures. Analysis of observed groundwater level fluctuations can provide an understanding of the connectivity between the aquifer, Biscayne Bay, and the water level in the canals. Further, groundwater level fluctuations can be used for aquifer parameter estimation. In this research, observed ocean water levels measured at tidal stations and groundwater levels are fitted to Jacob’s analytical solution, where the amplitude of the groundwater head fluctuation decreases exponentially, and the time lag increases with distance from the shore. Observed groundwater levels were obtained from monitoring wells along the Miami-Dade shore and the barrier island of Miami Beach. Results indicate that Jacob’s solution is effective for aquifer parameter estimation in Miami Beach, where monitoring wells are closer to the shore. Estimated hydraulic conductivity appears to increase by four orders of magnitude to approximately 1 m s^–1 as the distance from shore increases. Constructing monitoring wells closer to the shore in Miami-Dade County and elsewhere would permit improved aquifer parameter estimation and support enhanced groundwater modeling efforts.