| Biochemical, chromatographic and spectroscopic analyses of Suwannee River fulvic acid-Evidence for the presence of a DNA-like material |
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| 作者姓名： | Xiaodong MA Nawab ALI |
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| 作者单位： | Graduate Institute of Technology, University of Arkansas at Little Rock, 2801 S. Univ. Ave., Little Rock, AR 72204, USA |
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| 摘 要： | Naturally occurring organic compounds are complicated materials that are hard to fractionate into individual components. Organic materials from lakes, rivers and oceans often contain biological molecules related to proteins and DNA, and have global environmental and ecological importance. In this study, we used a combination of biochemical, chromatographic and spectroscopic methods to fractionate and characterize Suwannee River fulvic acid （SRFA） to determine if it contains proteins and DNA or DNA-like materials. A stock solution of SRFA （50 mg/mL） was prepared in double distilled deionized water. An initial biochemical analysis indicated that SRFA contained approximately 2% protein by weight, as determined by Bradford method using bovine serum albumin as standard. In order to determine the presence of DNA-like materials, a portion of the SRFA sample was extracted for DNA using chloroform/phenol extraction and ethanol precipitation method. When ethanol precipitated fraction was separated by agarose gel electrophoresis along with known DNA markers, a diffused ehthidium bromide staining was observed throughout the lane containing SRFA sample with a strong staining observed at the dye front, suggesting the presence of DNA or ＂DNA-like＂ molecules of various sizes in SRFA. To further fractionate, the DNA-like material on the gel was cut into seven fractions, each of which was extracted into aqueous phase. These fractions, along with the fractions obtained similarly from the original SRFA and an interface material during chloroform/ phenol extraction, were analyzed using UV-vis and fluorescence spectrometers, and LC/UV/MS. Comparisons of the absorption, fluorescence and mass spectra of various fractions revealed significant differences in their spectral properties. The DNA extracted materials showed stronger absorption in the DNA range of -260 nm than the original SRFA and interface material.
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| 关 键 词： | 河流 凝胶电泳法 色析法 荧光光谱 生物化学 水文化学 |
Biochemical, chromatographic and spectroscopic analyses of Suwannee River fulvic acid-Evidence for the presence of a DNA-like material |
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| Xiaodong MA Nawab ALI.Biochemical, chromatographic and spectroscopic analyses of Suwannee River fulvic acid-Evidence for the presence of a DNA-like material[J].Chinese Journal of Geochemistry,2006,25(B08):267-267. |
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| Abstract: | |
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| Keywords: | Suwannee River fulvic acid gel electrophoresis UV-vis and fluorescence spectroscopy LC/MS DNA-like |
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