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Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis
作者姓名:Gao Hongfeng  Cao Wen  Ji Minghou
作者单位:Institute of Oceanology, Academia Sinica, Qingdao 266071, China;Institute of Oceanology, Academia Sinica, Qingdao 266071, China;Institute of Oceanology, Academia Sinica, Qingdao 266071, China
摘    要:SubunitcompositionandchromophorecontentofR-phycocyaninandallophycocyaninfromPorphyrahaitanensis¥GaoHongfeng;CaoWendaandJiMing...

关 键 词:Porphyra  haitanensis  R-phycocyanin  allophycocyanin  subunit  and  chromophore
收稿时间:1993/2/26 0:00:00
修稿时间:1/8/1994 12:00:00 AM

Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis
Gao Hongfeng,Cao Wen,Ji Minghou.Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis[J].Acta Oceanologica Sinica,1994,13(2):253-259.
Authors:Gao Hongfeng  Cao Wenda and Ji Minghou
Institution:Institute of Oceanology, Academia Sinica, Qingdao 266071, China
Abstract:R-phycocyarnin (RPC) of Porphyra haitmmsis (T.J.Chang et B.F.Zheng) was chromatographed on Bio-Rex 70 column with urea solution (pH 3.0) as an eluent, and α and β two subunits were isolated.Their molecular weights were determined on SDS-PAGE at 18 400 and 20 500, respectively, while those of a and β subunits of allophycocyanin (APC) at 18 800 and 19 700, respeMively, and those of RPC and APC were at 117 000 and 122 000, respectively.
Both the molar ratio of α and β subunits of RPC and APC were 1:1, and the subunit composition was conf firmed to be(αβ)3.
It was ascertained that in RPC a subunit contains one chromophore phycocyanobilin (PCB) and β subunit has one chromophore PCB and one phycoerythrobilin (PEB),while in APC both α and β subunits contain one PCB.
Keywords:Porphyra haitanensis  R-phycocyanin  allophycocyanin  subunit and chromophore  
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