| 两个奥利亚罗非鱼群体热休克蛋白70基因序列比对分析 |
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| 引用本文： | 李莉萍,陈明,罗永巨,唐章生,唐瞻杨,陈忠,张永德,曾兰,林勇.两个奥利亚罗非鱼群体热休克蛋白70基因序列比对分析[J].广东海洋大学学报,2009,29(1). |
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| 作者姓名： | 李莉萍 陈明 罗永巨 唐章生 唐瞻杨 陈忠 张永德 曾兰 林勇 |
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| 作者单位： | 广西水产研究所,广西,南宁,530021 |
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| 基金项目： | 广西壮族自治区直属公益性科研院所基本科研业务费专项资金项目 |
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| 摘 要： | 采用RACE技术,对两个奥利亚罗非鱼群体(Oreochromis aurea)(美国奥利亚和中国奥利亚)热休克蛋白Hsp70基因完整编码区(code sequences,CDS)cDNA的进行克隆测序。序列分析结果表明:两个奥利亚罗非鱼群体热休克蛋白Hsp70基因CDS序列完全相同,全长1 923 bp,编码640个氨基酸,相对分子质量为70.29×103,理论等电点5.462,均具有Hsp70家族的3个签名序列:IDLGTTYS、IFDLGGGTFD、VVLVGGSTRIPKIQK;核定位信号标签KRKHKKDISQNKRALRR;Dank特征基序DLGTT-S-V;胞质Hsp70特征基序EEVD;靠近C端的GGMP4肽序列;2个糖基化位点NKSI和NVSA。对所得基因序列与已发表的青锵(Oryzias latipes)等物种Hsp70基因的氨基酸序列进行同源性比较,发现两个奥利亚罗非鱼群体与莫桑比克罗非鱼最高99.4%,与牙鲆最低83.9%;系统进化树分析表明奥利亚罗非鱼的Hsp70 cDNA序列与青鳉等物种的Hsp70基因聚在一支,而与牙鲆的Hsp70基因相分离。
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| 关 键 词： | 奥利亚罗非鱼 热休克蛋白70(Hsp70)基因 cDNA 克隆 序列分析 |
Sequence Comparison of Heat Shock Protein 70 Gene with Two Populations of Oreochromis aurea |
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| LI Li-ping,CHEN Ming,LUO Yong-ju,TANG Zhang-sheng,TANG Zhan-yang,CHEN Zhong,ZHANG Yong-de,ZENG Lan,LIN Yong.Sequence Comparison of Heat Shock Protein 70 Gene with Two Populations of Oreochromis aurea[J].Journal of Zhanjiang Ocean University,2009,29(1). |
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| Authors: | LI Li-ping CHEN Ming LUO Yong-ju TANG Zhang-sheng TANG Zhan-yang CHEN Zhong ZHANG Yong-de ZENG Lan LIN Yong |
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| Abstract: | Heat shock protein 70(Hsp70) gene was cloned and the sequences of the two populations of the blue tilapia Oreochromis aureas was analyzed by using rapid amplification cDNA ends(RACE).The results show that the sequences of Hsp70 are identical in the two populations of O.aureas.The Hsp70 gene contains an open reading frame of 1923bp which encodes 640 amino acid residues with an estimated relative molecular weight of 70.29×103 and a theoretical isoelectric point of 5.46.The deduced amino acid sequence of the Hsp70 gene has three signature sequences of the Hsp70 family: IDLGTTYS,IFDLGGGTFD and VVLVGGSTRIPKIQK,the sequence of bipartite nuclear targeting KRKHKKDISQ-NKRALRR,the sequence of dank subfamily(DLGTY-S-V),the sequence of cytoplasm EEVD,four peptide sequence GGMP close to C-extreme,and two glycosylation site(NKSI and NVSA).The deduced amino acid sequence of Hsp70 gene in O.aureas is very similar to O.mossambicus(99.4%) and Paralichthys olivaceus(83.9%).Phylogenetic tree analysis shows O.aureas is most closely related with Oryzias latipes and isolated from Paralichthys olivaceus. |
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| Keywords: | eDNA |
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