| 口蹄疫病毒VP1植物表达载体的构建 |
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| 引用本文： | 刘铀,毕英佐,马静云,曹永长.口蹄疫病毒VP1植物表达载体的构建[J].广东海洋大学学报,2004,24(4):59-62. |
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| 作者姓名： | 刘铀 毕英佐 马静云 曹永长 |
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| 作者单位： | 华南农业大学动物科学学院,广州,510642 |
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| 摘 要： | P1 T重组质粒上含有口蹄疫病毒 (FMDV)GD10分离株的p1cDNA片段 ,以此为模板 ,用PCR方法扩增其中的VP1基因 ,获得大小约 6 40bp的片段。该片段用BglⅡ和BstEⅡ酶切消化后克隆至表达载体 pCAMBIA130 5 .2 ,转化EcoliTOP10感受态细胞。重组质粒经PCR、酶切及序列分析 ,证实VP1基因处于CaMV35S启动子控制 ,且读码框正确
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| 关 键 词： | 口蹄疫病毒 VP1基因 植物表达载体 |
| 文章编号： | 1007-7995(2004)8-0059-04 |
| 修稿时间： | 2004年2月27日 |
Construction of VP1 Plant Expression Vector of Foot-and-Mouth Disease Virus |
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| LIU you,BI Ying-zuo,MA Jing-yun,CAO Yong-chang.Construction of VP1 Plant Expression Vector of Foot-and-Mouth Disease Virus[J].Journal of Zhanjiang Ocean University,2004,24(4):59-62. |
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| Authors: | LIU you BI Ying-zuo MA Jing-yun CAO Yong-chang |
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| Abstract: | The VP1 fragment with length of 639bp was amplified by PCR from P1-T plasmid containing the P1 cDNA segment of Foot-and-Mouth disease virus(FMDV) strain GD10. Being digested by BglII and BstEII, VP1 fragment was cloned into the expression vector pCAMBIA1305.2, Then it was transformed into Ecoli TOP10 competent cells. The recombined plasmid was detected by PCR,enzyme digestion and nucleotide sequencing. The result showed that the VP1 gene was inserted the correct ORF, under control of CaMV35S promotor. |
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| Keywords: | Foot-and-Mouth disease virus vp1 gene plant expression vector |
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