| 6-O-羧甲基壳多糖的细胞毒性效应研究 |
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| 摘 要： | 采用溶血试验和细胞毒性试验研究6 -O-羧甲基壳多糖的细胞毒性效应，结果表明：1% 浓度的6-O-羧甲基壳多糖对红细胞溶 血率小于5%，说明该材料无溶血现象。以手术缝合线和500μg/mL的苯酚为对照研究不同分 子量的6-O-羧甲基壳多糖分别在1000μg/mL和2000μg/mL浓度下对大白鼠皮肤成纤维细胞 生长的影响，经过2d，4d，7d细胞培养，细胞增殖率均大于100%，说明该材料对成纤维细胞 无毒性效应且对大白鼠皮肤成纤维细胞有促进生长的作用，分子量越小促生长作用越强，随 着培养时间延长促生长作用亦增强。此研究在一定程度上证明，6-O-羧甲基壳多糖作为医 用生物材料在细胞毒性方面是安全的。
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| 关 键 词： | 6-O羧甲基壳多糖 生物材料 细胞毒性 溶血 |
| 修稿时间： | 2000-01-10 |
Studies on the Cell Toxicity of 6-O-CM-Chitosan |
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| Authors: | Chen Xiguang |
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| Institution: | Chen Xiguang;(Ocean University of Qingdao,
Qingdao, 266003) |
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| Abstract: | The cell toxicity of 6-O-CM-Chitosan is studied by hemo lysis and cell toxicity experiments in this paper. The results show that the hem olysis of 6-O-CM-chitosan at the concentration of 1.0% is less than 5%, which means that 6-O-CM-chitosan has no hemolysis at this condition. The cell toxi city is studied by culturing mouse skin fibroblast in media containing 1000μg/m l and 2000μg/ml 6-O-CM-chitosan and contrasted with suture and 500μg/ml phe nol. The results demonstrate that the cell multiplication coefficient is more th an 100% after being cultured with 6-O-chitosan for 2d, 4d and 7d, which expres ses that 6-O-chitosan has no cell toxicity and can stimulate the proliferation of fibroblast obtained from the skin of mouse. The lower the molecule weight of 6-0-CM-chitosan is, and the longer the time the cells are exposed to 6-O-C M-chitosan, the greater the cell stimulation is. |
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| Keywords: | 6-O-CM-Chitosan biomaterial cell toxicity hemolys is |
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