Comparison of Chlorophyll a Concentrations Obtained with 90% Acetone and N,N-dimethylformamide Extraction in Coastal Seawater |
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| Authors: | Kuninao Tada Hitomi Yamaguchi Shigeru Montani |
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| Institution: | (1) Department of Life Sciences, Kagawa University, Miki, Kagawa 761-0795, Japan;(2) Graduate School of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan |
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| Abstract: | Determinations of Chlorophyll a (Chl a) in eutrophic coastal marine waters were compared using N, N-dimethylformamide (DMF) and 90% acetone techniques. Measured
Chl a concentrations ranged from 0.89 to 10.65 μg l−1 for 90% acetone extracts and from 0.97 to 12.92 μg l−1 for DMF extracts, respectively, for 24 surface water samples from the Harima-nada, Seto Inland Sea, Japan. Chl a concentrations using DMF as a solvent were consistently higher than those found using 90% acetone (p < 0.001, n= 24). Chl a is thus likely to be underestimated (by 13%) when 90% acetone is used for eutrophic waters.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | Chlorophyll a N N-dimethylformamide 90% acetone coastal water |
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