Mise au point d'un milieu de culture pour l'étude de l'altération de silicates en présence de Pseudomonas aeruginosa |
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| Authors: | Georges Aouad Valérie Geoffroy Jean-Marie Meyer Jean-Louis Crovisier Denis Damidot Peter Stille |
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| Institution: | 1. École et Observatoire des Sciences de la Terre, Centre de Géochimie de la Surface/CNRS UMR 7517, 1, rue Blessig, 67084 Strasbourg cedex, France;2. Laboratoire de Microbiologie et de Génétique, Université Louis-Pasteur/CNRS UMR 7156, 28, rue Goethe, 67083 Strasbourg cedex, France;3. Département « Génie Civil », École des Mines de Douai, 941, rue Charles-Bourseul, 59500 Douai cedex, France |
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| Abstract: | The influence of microorganisms on mineral alteration is not easy to determine in environmental conditions, because of the difficulty to raise for comparison purposes an identical but abiotic system. Another problem in this context is the choice of reliable tracers to evaluate the alteration rate of materials during in vitro experiments. To face such difficulties, we elaborated a defined medium allowing both the growth of Pseudomonas aeruginosa and a precise measurement of the elements solubilized from the minerals. Thanks to this medium, we were able to quantitatively determine the amounts of major elements solubilized from the materials in the presence of bacterial growth, compared to a sterile system. Moreover, the analysis by ICP-MS of trace elements was possible after a chromatographic treatment, which selectively eliminated 99% of the sodium content of the medium. To cite this article: G. Aouad et al., C. R. Geoscience 337 (2005). |
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| Keywords: | Milieu de culture Altération Biocorrosion Silicates complexes Bactéries Ciments Culture medium Alteration Biocorrosion Complex silicates Bacteria Cement |
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