| 鰶亚科两种鱼类的线粒体16S rRNA基因片段序列的比较研究 |
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| 引用本文： | 宋林,韩志强,高天翔.鰶亚科两种鱼类的线粒体16S rRNA基因片段序列的比较研究[J].海洋湖沼通报,2007(1):125-129. |
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| 作者姓名： | 宋林 韩志强 高天翔 |
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| 作者单位： | 1. 辽东学院,辽宁,丹东,118001
2. 中国海洋大学,教育部海水养殖重点实验室,山东,青岛,266003 |
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| 基金项目： | 国家高技术研究发展计划(中国近海鱼类资源的采集、保存与信息化技术)，国家重点基础研究发展计划(2005CB422306)资助 |
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| 摘 要： | 对鰶亚科两种鱼类的线粒体16S rRNA基因片段进行了PCR扩增和序列测定,分析比较了两种间的序列差异。斑鰶(Konosirus punctatus)16S rRNA基因片段长度为572bp,在3个个体中检测到2个单倍型,花鰶(Clupanodon thrissa)序列长度为575bp,两种间存在30个核苷酸位点(5.2%)的变异。基于木村双参数进化模型得到两种间的遗传距离为0.043。以太平洋鲱和寿南小沙丁为外群构建的NJ树表明,斑鰶和花鰶的亲缘关系较近,而与美洲真鰶(Dorosoma cepedianum)的亲缘关系较远。
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| 关 键 词： | 斑鰶 花鰶 16SrRNA基因 序列分析 |
| 文章编号： | 1003-6482（2007）01-0125-05 |
| 修稿时间： | 2006-08-27 |
SEQUENCE COMPARISON OF TWO DOROSOMATINAE SPECIES BASED ON PARTIAL 16 rRNA GENE SEQUENCE |
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| SONG Lin,HAN Zhiqiang,GAO Tianxiang.SEQUENCE COMPARISON OF TWO DOROSOMATINAE SPECIES BASED ON PARTIAL 16 rRNA GENE SEQUENCE[J].Transaction of Oceanology and Limnology,2007(1):125-129. |
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| Authors: | SONG Lin HAN Zhiqiang GAO Tianxiang |
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| Institution: | 1. Eastern Liaoning University, Dandong 118001, China;2. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China |
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| Abstract: | To evaluate the genetic variance of two Dorosomatinae fishes,mitochondrial 16S rRNA gene segment was amplified and sequenced.Two haplotypes were detected in three individuals of Konosirus punctatus.Of the 575 nucleotide sites in the 16S rRNA gene of K.punctatus and Clupanodon thrissa,30 sites(5.2%) were polymorphic.The k-2p genetic distance between the two species was 0.043.Based on 16S rRNA sequences,NJ phylogenetic tree using Clupea pallasi and Sardinella zunasi as outgroup showed a close genetic relationship between two Dorosomatinae species,and farther between them with Dorosoma cepedianum. |
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| Keywords: | Konosirus punctatus Clupanodon thrissa 16S rRNA gene sequence analysis |
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