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石油降解菌对石油烃的降解能力及影响因素研究
引用本文:白洁,崔爱玲,吕艳华.石油降解菌对石油烃的降解能力及影响因素研究[J].海洋湖沼通报,2007(3):41-48.
作者姓名:白洁  崔爱玲  吕艳华
作者单位:1. 海洋环境与生态教育部重点实验室,中国海洋大学,青岛,266100;环境科学与工程学院,中国海洋大学,青岛,266100
2. 环境科学与工程学院,中国海洋大学,青岛,266100
基金项目:中国典型滨海湿地污染控制与生物修复技术(2004040-1)资助
摘    要:采取油浓度梯度升高的方法,从胜利油田石油污染土壤中富集分离出以柴油为碳源和能源的优势除油混合菌B4。混合菌中分离出7株可培养菌。混合菌在含油0.5%的无机盐液体培养基中培养7天后柴油的去除率达到90.4%。该菌有较好的耐受性,当盐度达3.5时,油浓度达1.0%,亦有较好长势。混合菌对油污染土壤的修复有较好的效果,特别是加入营养盐,降解率达85.6%。混合菌脱氢酶动态变化的初步研究结果表明微生物活性变化与降解率有很好的相关性。

关 键 词:石油污染  混合菌  微生物降解
文章编号:1003-6482(2007)03-0041-08
修稿时间:2006-03-08

THE DEGRADATION CAPABILITY OF CRUDE OIL DEGRADING STRAINS AND THE IMPACTING FACTORS
BAI Jie,CUI Ailing,LV Yanhua.THE DEGRADATION CAPABILITY OF CRUDE OIL DEGRADING STRAINS AND THE IMPACTING FACTORS[J].Transaction of Oceanology and Limnology,2007(3):41-48.
Authors:BAI Jie  CUI Ailing  LV Yanhua
Institution:1. Ministry of education key laboratory of Marine Environmental Science and Ecology, Ocean University of China, Qingdao 266100; 2. College of Enviroment Science and Engineering, Ocean University of China, Qingdao 266100
Abstract:A bacterial consortium consisting of seven members has been collected after enrichment from the oil polluted soil ofShengli Oil Field using diesel fuel as carbon and energy sources through the gradient rise method of the density of oil.The consortium degraded 90.4% of diesel fuel after incudating 7 days in liquid culture.The consortium has better tolerance,and grows better when the salinity is up to 3.5% and the density of oil is up to 1.0%.Better results are achieved in the bioremediation of the oil pollution soil using the consortium,especially when nutrition salt is added,the degradation rate being up to 85.6%.According to the preliminary study of the dynamic change of dehydrogenase,the bacterial dehydrogenase activity is highly correlated with the degradation rate.
Keywords:oil pollution  consortium  biodegradation
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