| Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas： segregation and null allele identification for linkage analysis |
| |
| 引用本文： | 李莉,郭希明,张国范.Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas： segregation and null allele identification for linkage analysis[J].中国海洋与湖沼学报,2009,27(1). |
| |
| 作者姓名： | 李莉 郭希明 张国范 |
| |
| 作者单位： | LI Li,ZHANG Guofan(Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China);GUO Ximing(Haskin Shellfish Research Laboratory, Institute of Coastal and Marine Sciences, Rutgers University, New Jersey 08349, USA)
|
| |
| 摘 要： |
|
| 关 键 词： | 基因连锁分析 太平洋牡蛎 微卫星位点 鉴定 分离 微型 毛细管电泳 ABI公司 |
Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis |
| |
| LI Li,GUO Ximing,ZHANG Guofan,Key Laboratory of Experimental Marine Biology.Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas: segregation and null allele identification for linkage analysis[J].Chinese Journal of Oceanology and Limnology,2009,27(1). |
| |
| Authors: | LI Li GUO Ximing ZHANG Guofan Key Laboratory of Experimental Marine Biology |
| |
| Institution: | [1]Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Haskin Shellfish Research Laboratory. Institute of Coastal and Marine Sciences, Rutgers University, New Jersey 08349, USA |
| |
| Abstract: | Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping. |
| |
| Keywords: | Crassostrea gigas microsatellite null allele genetic mapping |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
