| CHLOROPHYLL FLUORESCENCE RATIO F685/F735 IN BROWN ALGAE AND ITS VARIATION UNDER EXCITATION BY TWO TYPES OF LIGHT AND DEHYDRATION |
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| 作者姓名： | 武宝玕 臧汝波 |
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| 作者单位： | Institute of Hydrobiology，Jinan University，Guangzhou 510632，Institute of Oceanology，Academia Sinicd，Qingdao 266071 |
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| 基金项目： | This work is supported by a fellowship of the EMBL(The Experimental Marine Biological Laboratory,Institute of Oceanology,Academia Sinica) |
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| 摘 要： | Calculation and comparative study of the chlorophyll fluorescence ratio F685/F735 in brown algae (Laminaria japonica, Underia pinnatifida and Padina crassa) excited by blue and green light showed that the fluorescence ratios were higher when the algae were excited by blue light (440 nm), but reduced obviously under green light (540 nm) excitation. The values also reduced under dehydration but could recover during rehydration if the stress was not serious. The variation of the fluorescence ratio under dehydration was mainly because changes in fluorescence emission at 735 nm were always sharper than those at 685 nm. The ratio was sensitive to stress and has potential as a stress indicator in phycological research. Measurement of the fluorescence excitation spectra showed that the only peak at 540 hm changed apparently during dehydration. It meant that the function of the Chl a /Fucoxanthin protein complex for energy transfer was easily inhibited by water stress. However, no variation of the ratio was foun
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| 收稿时间： | 21 February 1992 |
Chlorophyll fluorescence ratio F685/F735 in brown algae and its variation under excitation by two types of light and dehydration |
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| Wu Baogan,Zang Rubo.CHLOROPHYLL FLUORESCENCE RATIO F685/F735 IN BROWN ALGAE AND ITS VARIATION UNDER EXCITATION BY TWO TYPES OF LIGHT AND DEHYDRATION[J].Chinese Journal of Oceanology and Limnology,1993,11(1):1-7. |
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| Authors: | Wu Baogan Zang Rubo |
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| Institution: | (1) Institute of Hydrobiology, Jinan University, 510632 Guangzhou;(2) Institute of Oceanology, Academia Sinica, 266071 Qingdao |
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| Abstract: | Calculation and comparative study of the chlorophyll fluorescence ratio F685/F735 in brown algae (Laminaria japonica, Underia pinnatifida and Padina crassa) excited by blue and green light showed that the fluorescence ratios were higher when the algae were excited by blue light (440 nm), but reduced obviously under green light (540 nm) excitation. The values also reduced under dehydration but could recover during rehydration if the stress was not serious. The variation of the fluorescence ratio under dehydration was mainly because changes in fluorescence emission at 735 nm were always sharper than those at 685 nm. The ratio was sensitive to stress and has potential as a stress indicator in phycological research. Measurement of the fluorescence excitation spectra showed that the only peak at 540 hm changed apparently during dehydration. It meant that the function of the Chl a /Fucoxanthin protein complex for energy transfer was easily inhibited by water stress. However, no variation of the ratio was found in green alga Ulva pertusa under dehydration. Soil seemed to be something special for brown algae. The mechanism of this phenomenon is still unclear but may be related to the photosynthetic pigments and structural characteristics of brown algae. |
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| Keywords: | chlorophyll fluorescence ratio F685/F735 brown algae excitation |
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