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| 海洋链霉菌M506 的鉴定及代谢产物抑菌活性初步研究 | |
| 引用本文： | 朱本伟,李富超,秦 松.海洋链霉菌M506 的鉴定及代谢产物抑菌活性初步研究[J].海洋科学,2012,36(12):45-49. |
| 作者姓名： | 朱本伟 李富超 秦 松 |
| 作者单位： | 1. 中国科学院烟台海岸带研究所,山东烟台264003;中国科学院海洋研究所海洋生物学重点实验室,山东青岛266071;中国科学院研究生院,北京100049 2. 中国科学院海洋研究所海洋生物学重点实验室,山东青岛,266071 3. 中国科学院烟台海岸带研究所,山东烟台,264003 |
| 基金项目： | 国家自然科学基金项目(31000037); 中国科学院知识创新工程项目(KZCX2-YW-JC201); 山东省自然科学基金项目(JQ200914,ZR2009EQ004); 南通市科技项目(AS2011012) |
| 摘 要： | 自胶州湾沉积物中分离出海洋链霉菌M506,根据其形态特征观察、生理生化特征试验及基于16S rDNA序列系统进化分析,初步鉴定该菌为灰略红链霉菌.采用不同培养条件对其进行小规模发酵,利用金黄色葡萄球菌(Staphylococcus aureus),大肠杆菌(Escherichia coli),枯草芽孢杆菌(Bacillus subtilis)和苏云金芽孢杆菌(Bacillus thuringiensis)四株细菌作为指示菌,检测其抑菌活性.结果表明,该菌株代谢产物粗提物对于金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌等具有显著抑制活性. |
| 关 键 词： | 海洋链霉菌 抑菌活性 鉴定 |
| 收稿时间： | 1/9/2012 12:00:00 AM |
| 修稿时间： | 2012/4/23 0:00:00 |
Identification of marine Streptomyces sp. M506 and the antimicrobial activity of its metabolites |
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| ZHU Ben-wei,LI Fu-chao,QIN Song.Identification of marine Streptomyces sp. M506 and the antimicrobial activity of its metabolites[J].Marine Sciences,2012,36(12):45-49. | |
| Authors: | ZHU Ben-wei LI Fu-chao QIN Song |
| Institution: | 1(1.Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;2.Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;3.Graduate School of the Chinese Academy of Sciences,Beijing 100049,China) |
| Abstract: | Marine Streptomyces sp. M506 was isolated from the sediment of Jiaozhou Bay. According to morphological observations, physiological biochemical characteristics and 16S rDNA phylogenetic analysis, the strain M506 was identified to be Streptomyces griseorubens. In this study, the culture conditions had been optimized and six kinds of medium were used to culture the strain M506. The crude extracts from M2+ and SM medium exhibited distinct antimicrobial activities against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Bacillus thuringiensis. The result indicated that the culture condition optimization is an improved strategy to screen bioactive metabolites from marine Streptomyces. |
| Keywords: | Marine Streptomyces antimicrobial activity identification |
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