| Molecular Cloning and Expression ofPoIR2, a Novel Gene Involved in Immune Response in Japanese Flounder （Paralichthys olivaceus） |
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| 作者单位： | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China |
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| 摘 要： |
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| 关 键 词： | 免疫相关基因 免疫反应 克隆和表达 牙鲆 蛋白质结构分析 GenBank 分子 开放阅读框 |
| 收稿时间： | 27 December 2005 |
Molecular cloning and expression of <Emphasis Type="Italic">PoIR</Emphasis>2, a novel gene involved in immune response in Japanese flounder (<Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>) |
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| Authors: | Shuo Li Chunmei Li Xubo Wang Yanan Wang Zhipeng Liu Teng Zhai Quanqi Zhang |
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| Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China |
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| Abstract: | A novel immune-related gene was expressed in Japanese flounder (Paralichthys olivaceus) injected with Vibrio anguillarum. The complete cDNA contained a 169 bp 5’UTR, a 336 bp open reading frame (ORF) encoding 111 amino acids and a 556bp 3’UTR.
Six exons and five introns were identified in the PoIR2 gene. Blastp similarity comparison showed its encoding protein had 50% similarity to Danio rerio neuromedin S (NMS), but further alignment indicated they did not have NMS C-terminal conservational signature domain. So
it was not defined as an NMS homologue. Protein structure analysis indicated it had a 26aa signal peptide and was a secretory
pathway protein. RT-PCR demonstrated that the expression of PoIR2 was quickly induced and drastically increased in liver, kidney, spleen, gills, intestine, heart, and skeletal muscle after
infected with V. anguillarum. These results indicated that the PoIR2 might play some important role in Japanese flounder immune response system. This gene was named PoIR2 (P.olivaceus immune-related gene 2, GenBank accession number: EU224372). The mature PoIR2 peptide was expressed in BL21(DE3) pLysS using pET-32a(+) vector and a great part of the recombinant mature peptide existed
as soluble type. |
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| Keywords: | Paralichthys olivaceus Vibrio anguillarum immune-related gene (PoIR2) tissue expression prokaryotic expression |
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