Early stage differentiation of thallus cells ofPorphyra haitanensis (Rhodophyta) |
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| Authors: | Wang Sujuan Sun Yunlong Lu Anming Wang Guangyuan |
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| Institution: | (1) Shanghai Fisheries University, Shanghai, China;(2) Shanghai Institute of Plant Physiology, Shanghai, China |
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| Abstract: | The early stage differentiation of thallus cells ofPorphyra haitanensis T. J. Chang et B. F. Zheng was studied. Protoplasts or single cells were isolated from the blades using enzyme mixture comprising
2% sea snail gut enzyme and 1% cellulase. The isolated protoplasts or single cells were incubated in the MES medium. The cell
differentiations were examined under the microscope at intervals after incubation. Four types of cell differentiation, namely,
normal, abnormal, carposporangial and spermatorangial, and rhizoidal types, were observed. Since normal cell differentiations
occur mostly in small thalli 50 mm in length and middle portions of big thalli 200 mm in length, it is essential to select
tissues from these two kinds of thalli essential for commercial production. |
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