| Generation of Reactive Oxygen Species in Different Fractions of the Coelomocytes of Holothurian Eupentacta fraudatrix in Response to the Thermostable Toxin of Yersinia pseudotuberculosis in Vitro |
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| 作者姓名： | Dolmatova L.S. Eliseykina M.G. Timchenko N.F. Kovaleva A.L. Shitkova O.A. |
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| 作者单位： | [1]V.I.ll‘ichevPacificOceanologicalInstitute,Vladivostok690041,Russia [2]InstituteofMarineBiology,Vladivostok690041,Russia [3]ResearchInstituteofEpidemiologyandMicrobiology,Vladivostok690087,Russia |
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| 基金项目： | ThisworkwasfinanciallysupportedbytheRussionFoundationforFundamentalResearchGrant (No.0 0 0 4 48949) |
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| 摘 要： | Pure fraction (92% - 95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38 % )-FPMC- of the holothurian Eupentacta fraudatrix ( Holothuroidea,Dendrochirota ) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at differentconcentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in ccncentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 htreatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians.
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| 关 键 词： | 耶尔森氏菌属 热稳定 毒素 氧 噬菌细胞 阿米巴样细胞 桑椹胚细胞 海参 过氧化物 |
Generation of Reactive Oxygen Species in Different Fractions of the Coelomocytes of Holothurian Eupentacta fraudatrix in Response to the Thermostable Toxin of Yersinia pseudotuberculosis in Vitro |
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| Dolmatova,L.S. Eliseykina,M.G. Timchenko,N.F. Kovaleva,A.L. Shitkova,O.A..Generation of Reactive Oxygen Species in Different Fractions of the Coelomocytes of Holothurian Eupentacta fraudatrix in Response to the Thermostable Toxin of Yersinia pseudotuberculosis in Vitro[J].Chinese Journal of Oceanology and Limnology,2003,21(4):293-304. |
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| Authors: | Dolmatova LS Eliseykina M GTimchenko NF Kovaleva AL Shitkova OA |
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| Abstract: | |
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| Keywords: | bacterial toxin coelomocytes holothurian reactive oxygen species superoxide dismutase |
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