| PHYLOGENETIC RELATIONSHIPS AMONG PUFFERFISH OF GENUS TAKIFUGU BY RAPD ANALYSIS |
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| 作者姓名： | 宋林生 刘保忠 王在照 李红蕾 相建海 钱培元 |
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| 作者单位： | SONG Lin sheng，LIU Bao zhong，WANG Zai zhaoLI Hong lei，XIANG Jian hai(Institute of Oceanology，Chinese Academy of Sciences，Qingdao 266071，China |
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| 基金项目： | Thisresearchwassupportedby863HighTechnologyProject(No.81 9 0 4 0 7)fromtheChineseMinistryofScienceandTech
nologyandagrant(MFG |
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| 摘 要： | ＩＮＴＲＯＤＵＣＴＩＯＮＰｕｆｆｅｒｆｉｓｈｅｓｏｆｔｈｅｇｅｎｕｓＴａｋｉｆｕｇｕ (Ｔｅｔｒａｏｄｏｎｔｉｆｏｒｍｅｓ,Ｔｅｔｒａｏｄｏｎｔｉｄａｅ)ａｒｅｍａｉｎｌｙｄｉｓｔｒｉｂｕｔｅｄａｌｏｎｇｔｈｅｃｏａｓｔａｌｒｅｇｉｏｎｓｏｆＣｈｉｎａ ,ＪａｐａｎａｎｄＫｏｒｅａａｎｄａｒｅｃｏｍｍｅｒｃｉａｌｌｙｉｍｐｏｒｔａｎｔｔｏｔｈｅｓｅｃｏｕｎ ｔｒｉｅｓ .Ｏｖｅｒ 2 0ｓｐｅｃｉｅｓｈａｖｅｂｅｅｎｒｅｃｏｒｄｅｄｉｎｔｈｅＹｅｌｌｏｗＳｅａａｎｄｔｈｅＥａｓｔＣｈｉｎａＳｅａ (Ｃｈｅｎｇ…
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PHYLOGENETIC RELATIONSHIPS AMONG PUFFERFISH OF GENUS TAKIFUGU BY RAPD ANALYSIS |
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| SONG Lin sheng,LIU Bao zhong,WANG Zai zhaoLI Hong lei,XIANG Jian hai.PHYLOGENETIC RELATIONSHIPS AMONG PUFFERFISH OF GENUS TAKIFUGU BY RAPD ANALYSIS[J].Chinese Journal of Oceanology and Limnology,2001(2). |
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| Authors: | SONG Lin sheng LIU Bao zhong WANG Zai zhaoLI Hong lei XIANG Jian hai |
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| Abstract: | Amplifications with 300 10 base primers under predetermined optimal reaction yielded 2762 reproducible amplified fragments ranging from 200 to 3000 bp. Genetic distances among five species of Takifugu and Lagocephalus spadiceus as outgroup were calculated based on the presence/absence of the amplified fragments. Topological phylogenic trees of the 5 species of Takifugu and the outgroup were generated by Parsimony and Neighbor Joining analysis based on RAPD data set. The genetic distance between T. rubripes and T. pseudommus was almost the same as that between individuals within each species, but was much smaller than that among T. rubripes , T. pseudommus and the other species. The molecular data from RAPD analysis convincingly showed that T. rubripes and T. pseudommus should be the same species. |
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| Keywords: | Takifugu polymorphisms phylogeny RAPD |
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