| 普通野生稻中NBS-LRR类基因的克隆及Southern杂交 |
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| 引用本文： | 邢姗姗,胡汉桥,李信申,何红.普通野生稻中NBS-LRR类基因的克隆及Southern杂交[J].广东海洋大学学报,2007,27(3):112-116. |
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| 作者姓名： | 邢姗姗 胡汉桥 李信申 何红 |
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| 作者单位： | 广东海洋大学农学院,广东湛江,524088 |
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| 摘 要： | 利用已克隆植物的R基因NBS序列中保守基序设计简并引物进行PCR扩增是克隆NBS有效的方法。从广东普通野生稻HLW028中克隆出3条序列,经同源性分析均为NBS,序列号为DQ272573-DQ272575。从NCBI中下载普通野生水稻和功能已知的水稻NBS-LRR类基因,与本实验所提交的NBS进行聚类分析。这些NBS可分为5大类,其中DQ272574是一个新的NBS基因类型。从野生水稻中克隆NBS基因存在P-loop、RNBS-A、kin-2、RNBS-B、RNBS-C和PLAL的基序。Southern杂交结果表明,野生水稻HLW028和GZW035中具有与DQ272574同源的3个拷贝。
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| 关 键 词： | NBS-LRR 普通野生水稻 聚类分析 |
| 文章编号： | 1673-9159（2007）03-0002-05 |
| 修稿时间： | 2006-11-01 |
Cloning of NBS Gene from Wild Rice and Southern Blotting |
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| XING Shan-shan,HU Han-qiao,LI Xin-shen,HE Hong.Cloning of NBS Gene from Wild Rice and Southern Blotting[J].Journal of Zhanjiang Ocean University,2007,27(3):112-116. |
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| Authors: | XING Shan-shan HU Han-qiao LI Xin-shen HE Hong |
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| Institution: | Agricultural college of Guangdong Ocean university, Zhangjiang 524088,China |
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| Abstract: | It was an effective method to clone NBS by using the degenerate primers designed by the conserved motifs of NBS of the cloned R genes. Three sequences were cloned from wild rice HLW028 and were identified as NBS.The accession numbers were DQ272573-DQ272575.The NBS of wild rice and functionally known NBS of rice were downloaded.Phylogenetic analyses were conducted with the three submitted NBS and other downloaded NBS.The NBS could be divided into five groups,in which DQ272574 was a new kind of NBS.The submitted NBS contained the motifs such as P-loop,RNBS-A,kin-2,RNBS-B,RNBS-C and PLAL.The result of southern blotting showed that there were 3 copies of NBS which were homologous with DQ272574. |
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| Keywords: | NBS-LRR |
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