| 安络小皮伞［Marasmius androsaceus （L：Fr）Fr］中水溶性多糖的研究Ⅳ总糖及糖蛋白的分布、分级与测定 |
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| 引用本文： | 陈西广,张翼伸.安络小皮伞［Marasmius androsaceus （L：Fr）Fr］中水溶性多糖的研究Ⅳ总糖及糖蛋白的分布、分级与测定[J].中国海洋大学学报(自然科学版),1994(2). |
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| 作者姓名： | 陈西广 张翼伸 |
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| 作者单位： | 青岛海洋大学，东北师范大学 |
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| 摘 要： | 用水提取的安络小皮伞的多糖．经醇析．真空干燥后，用ｓｅｐｈａｒｏｓｅ４Ｂ柱层析，酚一Ｈ２ＳＯ４法检测其多糖分布，紫外吸收法（２８０ｎｎ）检测其蛋白质分布，表明提取物为多糖和糖蛋白的混合物，总糖含量为３０．５％。ｌｏｒｒｙ法和克氏定氮法测定蛋白质含量１５％。采用Ｐ．Ｃ和Ｇ．Ｃ法测定，结果提取物中的多糖由三种中性单糖组成．其摩尔比为：ＧＬＣ：Ｍａｎ，ＦＵＣ＝１．２：３：１．７。用Ｆｅｈｌｉｎｇ试剂法测定，表明混合物中有甘露聚精。Ｆｅｌｌｌｉｎｇ试剂法与甲醇、乙醇分级法相结合，从提取物中分出六个糖蛋白级分：Ｆｐ１、Ｆｐ２、Ｆｓ１、Ｆｓ２、Ｆｓ３和Ｆｓ４，其中Ｆｐ１、Ｆｓ１和Ｆｓ３经ｓｅｐｈａｒｏｓｅ４Ｂ柱测定为多糖分子量单一的级分。
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| 关 键 词： | 安络小皮伞 安络小皮伞多糖 糖蛋白 |
STUDY ON WATER SOLUBLE POLYSACCHARIDE FROM MARASMIUS ANDROSACEUS(L: Fr)FR. IV DISTRIBUTION,GRADATION AND DETERMINATION OF THE RUDE POI-YSACCHARID AND GI-YCOPROTEIN |
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| Chen Xiguang.STUDY ON WATER SOLUBLE POLYSACCHARIDE FROM MARASMIUS ANDROSACEUS(L: Fr)FR. IV DISTRIBUTION,GRADATION AND DETERMINATION OF THE RUDE POI-YSACCHARID AND GI-YCOPROTEIN[J].Periodical of Ocean University of China,1994(2). |
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| Authors: | Chen Xiguang |
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| Institution: | Chen Xiguang(Ocean University of Qingdao) Zhang Yishen(Northeasten Nomal University) |
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| Abstract: | The water soluble rude polysaccharide was isolated from Mirasmius androsaceus. It contained 30. 59% of carbohydrate and 15% of protein. The sugar molar ratio determined by P. C. and G. C. was Glc: Man 2 Fuc=1. 2: 3. 0: 1. 7.It was graded by Fehling reagent to isolate the mannans (FP) and the heteropolysaccharides(Fs). The molar ratio of sugar in Fs was GLC: Man: Fuc= 1. 4 : 1. 0: 6. 6. Fp and Fs were purified with Mathanol and Ethanol respectively.With this method we obtained six fractions (Fp1, Fp2, Fs1, Fs2, Fs3, Fs4), half of which were homogeneous polysaccharide. |
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| Keywords: | Marasmius androsaceus Mgrasmius polysaccharides: Glycoprotein |
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