| In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus |
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| 作者姓名： | 宿烽 张士璀 李红岩 郭华荣 |
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| 作者单位： | [1]Department of Bioengineering ＆Pharmaceutics, Qingdao University of Science ＆ Technology, Qingdao 266042, China [2]Department of Marine Biology, Ocean University of China, Qingdao 266003, China |
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| 基金项目： | the Ministry of Science and Technology (MOST) of China (No. 2001AA649040)，the Scientific-Research Fund of Qingdao University of Science and Technology (No.0022168) |
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| 摘 要： | In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid (IMI) to the gill cell line of flounder (FG) that collected in the gill of Paralichthys olivaceus, was examined by 3 widely used endpoint bioassays: NR (neutral red), MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and TCP (total cell protein). The result shows that the IMI increased at concentrations ≥0.5 μg/ml. The IC50 value of NR, MTT, and TCP was 41.86, 38.46, and 39.08 μg/ml, respectively. The ultrastructural observation revealed that the mitochondria of the cells exposed to 60 μg/ml IMI for 48 h were severely damaged, swollen or disrupted, while their nuclei and rough endoplasmic reticulum (RER) remained normal. This would suggest that the mitochondria are probably the primary target of IMI.
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| 关 键 词： | 吡虫啉 杀虫剂 比目鱼 鳃部细胞系 急性毒性 |
| 收稿时间： | 2006-03-13 |
| 修稿时间： | 2006-05-10 |
<Emphasis Type="Italic">In vitro</Emphasis> acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder <Emphasis Type="Italic">Paralichthy olivaceus</Emphasis> |
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| Su Feng,Zhang Shicui,Li Hongyan,Guo Huarong.In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus[J].Chinese Journal of Oceanology and Limnology,2007,25(2):209-214. |
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| Authors: | Su Feng Zhang Shicui Li Hongyan Guo Huarong |
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| Institution: | (1) Department of Bioengineering & Pharmaceutics, Qingdao University of Science & Technology, Qingdao, 266042, China;(2) Department of Marine Biology, Ocean University of China, Qingdao, 266003, China |
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| Abstract: | In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid (IMI) to the gill cell line of flounder (FG) that collected
in the gill of Paralichthys olivaceus, was examined by 3 widely used endpoint bioassays: NR (neutral red), MTT (3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium
bromide) and TCP (total cell protein). The result shows that the IMI increased at concentrations ≥0.5 μg/ml. The IC50 value of NR. MTT, and TCP was 41.86, 38.46, and 39.08 μg/ml, respectively. The ultrastructural observation revealed that
the mitochondria of the cells exposed to 60 μg/ml IMI for 48 h were severely damaged, swollen or disrupted, while their nuclei
and rough endoplasmic reticulum (RER) remained normal. This would suggest that the mitochondria are probably the primary target
of IMI.
Supported by the Ministry of Science and Technology (MOST) of China (No. 2001AA649040) and the Scientific-Research Fund of
Qingdao University of Science and Technology (No.0022168) |
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| Keywords: | imidacloprid cell line cytotoxicity flounder Paralichthy olivaceus |
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