| 缢蛏微卫星序列分离及特性分析 |
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| 引用本文： | 牛东红,李家乐,郑润玲.缢蛏微卫星序列分离及特性分析[J].中国海洋大学学报(自然科学版),2008,38(5). |
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| 作者姓名： | 牛东红 李家乐 郑润玲 |
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| 作者单位： | 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海,200090 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，上海市重点学科建设项目 |
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| 摘 要： | 分析了缢蛏(Sinonovacula constricta)基因组文库中微卫星序列的特性及微卫星引物的遗传特征.采用磁珠富集珐和PCR 筛选法,以CA15为探针,快速分离含有微卫星序列的阳性克隆.结果表明,在筛选的210个白色菌落中,得到58个微卫星序列,阳性克隆率为27.6%,核心重复序列为两碱基和三碱基的微卫星居多,其中完美型占39.7%,非完美型39.7%,复合型占10.3%,非完美型与复合型共存的占10.3%.微卫星重复次数主要集中在20～40次之间,占66%,最高为63次.除探针中使用的CA重复单元外,还观察到AG/TC,AT/TA,AAC/TTG,CAA/GTT,TGA,AAT,AGAA,GTTT/CAAA,AAAT/TTTA,TTTC,CCTA,TCTA,TCCC,TGCC,GTTTCT,CTGTT,CACACC,TATACA,TTTTG的重复序列.利用Primer Premier5.0设计引物24对,经过初步筛选得到适合的微卫星引物18对,为今后未知微卫星标记的开发,群体遗传结构的分析,优良品种的选育提供基础.
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| 关 键 词： | 缢蛏 微卫星 磁珠富集 |
Isolation and Sequence Characterization of Microsatellite DNA in Razor Clam(Sinonovacula constricta) |
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| NIU Dong-Hong,LI Jia-Le,ZHENG Run-Ling.Isolation and Sequence Characterization of Microsatellite DNA in Razor Clam(Sinonovacula constricta)[J].Periodical of Ocean University of China,2008,38(5). |
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| Authors: | NIU Dong-Hong LI Jia-Le ZHENG Run-Ling |
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| Institution: | NIU Dong-Hong,LI Jia-Le,ZHENG Run-Ling(The Key Laboratory of Exploration , Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 200090,China) |
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| Abstract: | The microsatellite sequences and microsatellite primers were analyzed in razor clam(Sinonovacula constricta).The positive clones of microsatellites were isolated using the magnetic bead hybridization selection method and PCR selection method with the(CA)15 prone.A total of 210 white colonies were screened using a PCR-based technique,and there were 58 microsatellite repeat motifs by sequencing(27.6% of white colonies screened).Besides the motif of CA contained in the oligoprobe,we also found other 19 types o... |
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| Keywords: | PCR |
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