| 皱纹盘鲍脂肪酸及脂肪醛二甲基缩醛的气相色谱/质谱分析 |
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| 引用本文： | 楼乔明,王玉明,杨延存,高壮,薛长湖,贾敏.皱纹盘鲍脂肪酸及脂肪醛二甲基缩醛的气相色谱/质谱分析[J].中国海洋大学学报(自然科学版),2011,41(6):41-44,50. |
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| 作者姓名： | 楼乔明 王玉明 杨延存 高壮 薛长湖 贾敏 |
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| 作者单位： | 中国海洋大学食品科学与工程学院,山东,青岛,266003 |
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| 基金项目： | 海洋公益性行业科研专项，"泰山学者"建设工程专项经费资助 |
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| 摘 要： | 研究了用气相色谱/质谱法测定皱纹盘鲍中脂肪酸及脂肪醛二甲基缩醛的方法。皱纹盘鲍经Folch法提取总脂,用10%硫酸-甲醇溶液进行反应,通过气相色谱/质谱法对其脂肪酸及脂肪醛二甲基缩醛的组成进行分析。实验结果表明:10%硫酸-甲醇溶液能有效地对脂肪酸进行甲酯化衍生;同时促使烯醚键断裂,并与甲醇发生缩醛反应生成脂肪醛二甲基缩醛。通过气相色谱/质谱分析,从皱纹盘鲍中共鉴定出32个化合物,包括26种脂肪酸和6种脂肪醛二甲基缩醛;脂肪酸以C16:0、C20:4(n-6)、C20:5(n-3)、C18:1(n-7)和C22:5(n-3)为主;脂肪醛二甲基缩醛以C18:0DMA和C20:1DMA为主。皱纹盘鲍肌肉和内脏中的脂肪酸及脂肪醛二甲基缩醛的组成基本一致,但内脏中的4,8,12-三甲基十三烷酸含量远高于肌肉,具有极显著差异(P<0.01)。
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| 关 键 词： | 皱纹盘鲍 脂肪酸 脂肪醛二甲基缩醛 气相色谱/质谱法 |
Analysis of Fatty Acids and Fatty Aldehyde Dimethyl Acetals in Haliotis discus hannai Ino by Gas Chromatography/Mass Spectrometry |
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| LOU Qiao-Ming,WANG Yu-Ming,YANG Yan-Cun,GAO Zhuang,XUE Chang-Hu,JIA Min.Analysis of Fatty Acids and Fatty Aldehyde Dimethyl Acetals in Haliotis discus hannai Ino by Gas Chromatography/Mass Spectrometry[J].Periodical of Ocean University of China,2011,41(6):41-44,50. |
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| Authors: | LOU Qiao-Ming WANG Yu-Ming YANG Yan-Cun GAO Zhuang XUE Chang-Hu JIA Min |
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| Institution: | LOU Qiao-Ming,WANG Yu-Ming,YANG Yan-Cun,GAO Zhuang,XUE Chang-Hu,JIA Min(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | The determination of fatty acids and fatty aldehyde dimethyl acetals in Haliotis discus hannai Ino was studied using gas chromatography/mass spectrometry(GC/MS).The total lipids of H.discus hannai Ino were extracted using Folch method,derivatised with 10% H2SO4-CH3OH solution,and finally the composition of fatty acids and fatty aldehyde dimethyl acetals was analysed by GC/MS.The results showed that 10% H2SO4-CH3OH solution could prompt methyl esterification effectively,and made the breaking of vinyl ether b... |
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| Keywords: | Haliotis discus hannai Ino fatty acids fatty aldehyde dimethyl acetals gas chromatography/mass spestrometry(GC/MS) |
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