| SRAP标记在马氏珠母贝家系F1代中的分离 |
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| 引用本文： | 张红玉,何毛贤.SRAP标记在马氏珠母贝家系F1代中的分离[J].海洋通报,2009,28(2). |
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| 作者姓名： | 张红玉 何毛贤 |
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| 作者单位： | 1. 中国科学院南海海洋研究所,LMB,LAMB,广东,广州,510301;中国科学院研究生院,北京,100039
2. 中国科学院南海海洋研究所,LMB,LAMB,广东,广州,510301 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，科技支撑计划 |
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| 摘 要： | 用SRAP(sequence-related amplified polymorphism,序列相关扩增多态性)标记首次对马氏珠母贝(Pinctada martensii)红壳家系的亲本及其子一代(F1)进行了遗传分析.7对引物共扩增出63个位点,多态位点比例为68.25%,其中正常位点59个(93.65%),异常位点4个(6.35%).59个正常位点中,20个位点在F1不分离,39个位点分离.卡方检验表明,39个分离位点中符合孟德尔分离规律的位点28个,占分离位点数的71.79%;偏孟德尔分离规律的位点11个,占分离位点数的28.21%.符合孟德尔分离规律的位点中按1:1分离的位点占25%,3:1分离的位点占75%,1:1分离位点所占比例较小,因此要利用马氏珠母贝家系F1进行SPAP遗传图谱的绘制应适当增加引物数量或改用杂交家系.
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| 关 键 词： | 分离方式 SRAP标记 马氏珠母贝 |
Segregation Pattern of SRAP Maker in F1 Generation of Pinctada martensii Family |
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| ZHANG Hongyu,HE Maoxian.Segregation Pattern of SRAP Maker in F1 Generation of Pinctada martensii Family[J].Marine Science Bulletin,2009,28(2). |
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| Authors: | ZHANG Hongyu HE Maoxian |
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| Institution: | 1.LMB;LAMB;South China Sea Institute of Oceanology;Chinese Academy of Sciences;Guangzhou 510301;Guangdong;China;2.Graduate school;Beijing 100039;China |
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| Abstract: | The polymorphism and segregation of SRAP marker in the F1 generation of Pinctada martensii family with red-color shells were investigated using 7 primer combinations for the first time.Of the total 63 loci amplified,43 were polymorphic,accounting for 68.25 %;59 loci were normal,accounting for 93.65 %,and four loci were unexpectedly amplified in F1 but not in parents.Among the normal loci,39 were polymorphic in F1 generation,and 20 loci were monomorphic.Of the 39 segregating loci,11 loci(28.21 %) segregated ... |
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| Keywords: | F1 |
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