| In Silico Screening for Microsatellite Markers from Expressed Sequence Tags of Porphyra yezoensis （Bangiales,Rhodophyta） |
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| 作者姓名： | WANG Mengqiang HU Jingjie ZHUANG Yunyun ZHANG Lei LILT Wei MAO Yunxiang |
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| 作者单位： | College of Marine Life Science, Ocean University of China, Qingdao 266003, P. R. China |
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| 基金项目： | 国家高技术研究发展计划(863计划);农业部资助项目 |
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| 摘 要： | The genomic resources of Porphyra yezoensis expressed sequence tags （ESTs） were utilized to identify simple sequence repeats （SSRs）, or microsatellites. This method took the advantage of using ESTs and microsatellites either for the establishment of gene identities or for the acquisition of high polymorphism. The microsatellites can be used as gene markers when microsatellites are tagged to genes. Revealed by bioinformatics analysis, 1162 out of 21954 ESTs contained microsatellites and cluster analysis indicated that 984 of these ESTs fell into 112 contigs, while the other 178 ESTs were singletons. A total of 290 unique SSR-containing genes were identified. The AAC SSRs were the most populous type of microsatellites. GC-rich microsatellites were predominant among all the microsatellites.
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| 关 键 词： | 条斑紫菜 表达序列标签 微卫星标记 虚拟筛选 |
| 收稿时间： | 2006-08-17 |
| 修稿时间： | 2007-03-08 |
<Emphasis Type="Italic">In Silico</Emphasis> screening for microsatellite markers from expressed sequence tags of <Emphasis Type="Italic">Porphyra yezoensis</Emphasis> (Bangiales,Rhodophyta) |
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| WANG Mengqiang HU Jingjie ZHUANG Yunyun ZHANG Lei LILT Wei MAO Yunxiang.In Silico screening for microsatellite markers from expressed sequence tags of Porphyra yezoensis (Bangiales,Rhodophyta)[J].Journal of Ocean University of China,2007,6(2):161-166. |
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| Authors: | Wang Mengqiang Hu Jingjie Zhuang Yunyun Zhang Lei Liu Wei Mao Yunxiang |
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| Institution: | College of Marine Life Science, Ocean University of China, Qingdao 266003, P. R. China |
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| Abstract: | The genomic resources of Porphyra yezoensis expressed sequence tags (ESTs) were utilized to identify simple sequence repeats (SSRs), or microsatellites. This method took the advantage of using ESTs and microsatellites either for the establishment of gene identities or for the acquisition of high polymorphism. The microsatellites can be used as gene markers when microsatellites are tagged to genes. Revealed by bioinformatics analysis, 1 162 out of 21 954 ESTs contained microsatellites and cluster analysis indi- cated that 984 of these ESTs fell into 112 contigs, while the other 178 ESTs were singletons. A total of 290 unique SSR-containing genes were identified. The AAC SSRs were the most populous type of microsatellites. GC-rich microsatellites were predominant among all the microsatellites. |
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| Keywords: | bioinformatics data mining EST microsatellites Porphyra yezoensis |
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