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用含氯化镉培养液培养塔胞藻的生物学效应
引用本文:张建民,王刚,苗艳丽,昝金冬,马庆林,王丛丛.用含氯化镉培养液培养塔胞藻的生物学效应[J].台湾海峡,2004,23(1):51-55.
作者姓名:张建民  王刚  苗艳丽  昝金冬  马庆林  王丛丛
作者单位:山东大学威海分校海洋生物系,山东,威海,264209
摘    要:在塔胞藻培养液中加入不同浓度的CdCl2,然后分析了培养在各种处理和对照组的塔胞藻后代.结果发现,随着镉离子浓度的升高,细胞的生长繁殖量和体积在前2轮(9d为一轮)培养中均明显降低,而在第3轮培养时,细胞的繁殖和大小接近常态.随后对处理后多种细胞内含物含量进行了分析,显示出:随着培养液中镉离子浓度增高,细胞内的蛋白质、叶绿素含量均明显下降,而可溶性糖、核酸的含量与对照相比变化不大.

关 键 词:细胞生物学  生物学效应  CdCl2  塔胞藻
文章编号:1000-8160(2004)01-0051-05

Study on biologic effects of culturingPyramimonas sp.in medium containing CdCl2
ZHANG Jian-min,WANG Gang,MIAO Yan-li,ZAN Jin-dong,MA Qing-lin,WANG Cong-cong.Study on biologic effects of culturingPyramimonas sp.in medium containing CdCl2[J].Journal of Oceanography In Taiwan Strait,2004,23(1):51-55.
Authors:ZHANG Jian-min  WANG Gang  MIAO Yan-li  ZAN Jin-dong  MA Qing-lin  WANG Cong-cong
Abstract:This paper studied biologics effect of culturing the posterityof Pyramimonas sp. with various treatments by adding different concentrationCdCl_2 in themedium. The result showed that the reproduction and size of cell were obviously lower in the first and second rounds (9d/round), but they were near to normal state at the third round. The analysis of microalgae cell contents handled with cadmium ion showed that along with the increase of cadmium ion concentration insidemedium, the contents of protein and chlorophyll in the cell obviously decreased,while the changes of soluble sugar and nucleic acid contents in the cell were not so obvious.
Keywords:cell biology  biologic effects  CdCl_2  Pyramimonas sp  
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