Optimization of nutrient component for diesel oil degradation by Rhodococcus erythropolis |
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| Authors: | Huang Lei Ma Ting Li Dan Liang Feng-lai Liu Ru-Lin Li Guo-qiang |
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| Institution: | a The School of Chemistry and Chemical Engineering, Tianjin University of Technology, 263 Hongqi South Road, Tianjin 300191, China
b Tianjin Key Laboratory for Microbial Functional Genomics, Key Laboratory of Molecular Microbiology and Technology Ministry of Education, College of Life Sciences, Nankai University, Weijin Road 94, Tianjin 300071, China |
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| Abstract: | A novel bacterium T7-2 was isolated from the oil-polluted sea-bed mud of Bohai Sea, northern China, which can degrade diesel oil at 15 °C. This bacterium was identified as a strain of Rhodococcus erythropolis according to its 16S rDNA gene. In order to enhance degradation efficiency, a five-level, three-factor central composite design was employed to optimize the nutrition supplied to artificial seawater. The results indicate that a supplement of 2.53 g (NH4)2SO4 L−1, 2.75 g Na2HPO4 L−1 and 0.01 g yeast extract L−1 to artificial seawater increases the degradation rate from 12.61% to 75% within 7 d. |
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| Keywords: | Marine pollution Nutrient Oil degradation Optimization Response surface methodology Rhodococcus erythropolis |
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