| 狄氏斧蛤(Donax dysoni)线粒体全基因组测定及结构特征分析 |
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| 引用本文： | 马颜雯,李继姬,叶莹莹.狄氏斧蛤(Donax dysoni)线粒体全基因组测定及结构特征分析[J].海洋与湖沼,2023,54(1):246-258. |
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| 作者姓名： | 马颜雯 李继姬 叶莹莹 |
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| 作者单位： | 浙江海洋大学 国家海洋设施养殖工程技术研究中心 浙江舟山 316022 |
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| 基金项目： | 浙江省省属高校基本科研业务费,2021J005号;舟山市科技计划项目,2021C21017号。 |
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| 摘 要： | 为解析狄氏斧蛤(Donax dysoni)线粒体全基因组结构特征以及进化地位,采用二代高通量测序技术获得狄氏斧蛤线粒体基因组全序列,对线粒体基因进行注释并对其序列结构进行分析。结果表明,狄氏斧蛤的线粒体全基因组序列全长为16 908 bp,碱基组成为A (26.81%)、T (41.13%)、G(21.21%)和C (10.85%),A+T含量为67.94%,表现出明显的AT偏向。与其他双壳贝类相似,狄氏斧蛤共编码13个蛋白质编码基因,包含22个t RNA和2个r RNA,且所有基因均位于H链;蛋白质编码基因拥有3种起始密码子(ATG、ATT、ATA),而除了ND3、ND4以及ATP6使用TAG作为终止密码子外,其余所有蛋白质编码基因都使用TAA作为终止密码子。除t RNASer不能折叠成典型的三叶草结构,没有明显的DHU茎环,其余t RNA都能折叠成典型的三叶草结构。狄氏斧蛤与斧蛤科(Donacidae)其他物种具有相同的基因排列,未发现基因重排现象。基于线粒体12个蛋白质编码基因构建62种贝类的进化树,结果显示:Donax semiestriatus和Donax vittatus聚...
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| 关 键 词： | 狄氏斧蛤 线粒体基因组 基因重排 系统发育分析 |
| 收稿时间： | 2022/4/19 0:00:00 |
| 修稿时间： | 2022/7/7 0:00:00 |
COMPLETE SEQUENCE AND GENE ORGANIZATION OF THE MITOCHONDRIAL GENOME OF CLAM DONAX DYSONI |
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| MA Yan-Wen,LI Ji-Ji,YE Ying-Ying.COMPLETE SEQUENCE AND GENE ORGANIZATION OF THE MITOCHONDRIAL GENOME OF CLAM DONAX DYSONI[J].Oceanologia Et Limnologia Sinica,2023,54(1):246-258. |
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| Authors: | MA Yan-Wen LI Ji-Ji YE Ying-Ying |
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| Institution: | National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China |
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| Abstract: | To improve the systematics and taxonomy of Donax dysoni, the complete mitochondrial genome of D. dysoni was sequenced and annotated by the Next-Generation Sequencing. Results show that the total length of the mitochondrial genome is 16 908 bp, consisting A (26.81%), T (41.13%), G (21.21%) and C (10.85%), and A+T content was 67.94%, showing obvious AT bias. Similar to other bivalves, D. dysoni contains 13 proteins, 22 tRNAs, and 2 rRNAs genes, and all the genes are encoded by the H-strand. Protein-coding genes have three start codons (ATG, ATT, and ATA), and all protein-coding genes use TAA as the stop codon; except for ND3, ND4 and ATP6, they use TAG as the stop codon. Among the 22 tRNA genes, 21 tRNA genes (except only for tRNASerI) can fold into a typical perfect secondary structure due to miss a distinct DHU stem ring. The gene arrangements of all species of the Donacidae are consistent. The phylogenetic tree of 62 species show that Donax semiestriatus and Donax vittatus cluster into a small clade, and Donax trunculus and Donax variegatus cluster into a small clade, then clustered together with D. dysoni into Donacidae. The phylogenetic tree supports the inclusion of the family Donacidae to the Tellinoidea, and the inclusion of the order Cardiida to which the Tellinoidea belongs as a separate order rather than as part of the order Venerida. |
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| Keywords: | Donax dysoni mitochondrial genome gene rearrangement phylogenetic analysis |
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