Development of an inactivated iridovirus vaccine against turbot viral reddish body syndrome |
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| Authors: | Tingjun Fan Xiuzhong Hu Liyan Wang Xiaofen Geng Guojian Jiang Xiuxia Yang Miaomiao Yu |
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| Institution: | (1) Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, 266003 Qingdao, China |
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| Abstract: | Turbot (Scophthalmus maximus L.) reddish body iridovirus (TRBIV) was propagated in turbot fin cells (TF cells) and inactivated as the TRBIV vaccine with
its protection efficiency evaluated in this study. TF cells were cultured in 10% bovine calf serum (BCS)-containing MEM medium
(pH7.0) at 22°C, in which TRBIV propagated to a titer as high as 105.6 TCID50 mL−1. The TRBIV was inactivated with 0.1% formalin and formulated with 0.5% aluminum hydroxide. The inactivated vaccine caused
neither cytopathogenic effect (CPE) on TF cells nor pathogenic effect on turbots. After being administered with the vaccine
twice via muscle injection, the turbot developed high-tittered TRBIV neutralizing antibodies in a dose-dependent manner. The vaccine
protected the turbot from dying with an immunoprotection rate of 83.3% as was determined via subcutaneous vaccination in the laboratory and 90.5% via bath vaccination in turbot farms, respectively. The inactivated vaccine was very immunogenic, efficiently preventing turbot
from death. It holds the potential of being applied in aquaculture. |
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| Keywords: | turbot reddish body iridovirus turbot fin cell inactivated vaccine Scophthalmus maximus |
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