| 响应面法优化双髻鲨（Sphyrna zygaena）肌肉盐溶蛋白的提取和凝胶形成条件 |
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| 引用本文： | 徐大伦,颜伟华,杨文鸽,何斌辉,丁 宁,陈 丹,顾玲芳.响应面法优化双髻鲨（Sphyrna zygaena）肌肉盐溶蛋白的提取和凝胶形成条件[J].海洋与湖沼,2011,42(2):289-293. |
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| 作者姓名： | 徐大伦 颜伟华 杨文鸽 何斌辉 丁 宁 陈 丹 顾玲芳 |
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| 作者单位： | 宁波大学 应用海洋生物技术教育部重点实验室;宁波大学 应用海洋生物技术教育部重点实验室;宁波大学 应用海洋生物技术教育部重点实验室;宁波大学 应用海洋生物技术教育部重点实验室;宁波大学 应用海洋生物技术教育部重点实验室;宁波大学 应用海洋生物技术教育部重点实验室;宁波大学 应用海洋生物技术教育部重点实验室 |
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| 基金项目： | 国家“十一五”科技支撑计划重大项目, 2008BAD94B05 号; 宁波市自然科学基金项目, 2010A610013 号; 浙江省大学生科技创新基金项目, 2009—2010 |
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| 摘 要： | 采用响应面分析法,以双髻鲨肉盐溶蛋白凝胶保水性为依据,对盐溶蛋白的提取和凝胶形成条件NaCl浓度、pH和低温加热时间进行优化。结果表明：（1）根据中心组合试验设计,采用三因素三水平的响应面分析法,建立了双髻鲨肉盐溶蛋白凝胶保水性与其提取条件的数学模型：保水性（%）=65.440＋10.868X1＋1.835X2-0.5...
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| 关 键 词： | 双髻鲨 盐溶蛋白 凝胶保水性 响应面法 |
| 收稿时间： | 2010/1/28 0:00:00 |
| 修稿时间： | 2010/3/26 0:00:00 |
RESPONSE SURFACE OPTIMIZATION OF CONDITIONS FOR EXTRACTION AND GEL PREPARATION OF SALT-SOLUBLE PROTEINS FROM SPHYRNA ZYGAENA MUSCLE |
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| XU Da-Lun,YAN Wei-Hu,YANG Wen-Ge,HE Bin-Hui,DING Ning,CHEN Dan and GU Ling-Fang.RESPONSE SURFACE OPTIMIZATION OF CONDITIONS FOR EXTRACTION AND GEL PREPARATION OF SALT-SOLUBLE PROTEINS FROM SPHYRNA ZYGAENA MUSCLE[J].Oceanologia Et Limnologia Sinica,2011,42(2):289-293. |
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| Authors: | XU Da-Lun YAN Wei-Hu YANG Wen-Ge HE Bin-Hui DING Ning CHEN Dan and GU Ling-Fang |
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| Institution: | Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University;Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University |
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| Abstract: | Based on the Box-Benhnken experimental design with 3 factors and 3 levels, the effects of NaCl concentration (X1), pH (X2) and low-temperature heating time (X3) on the water holding capacity (WHC) of the salt-soluble protein gel from Sphyrna zygaena muscle were studied and optimized by the respond surface methodology (RSM). The correlation between the WHC of the salt-soluble protein gel and its extractive conditions was WHC(%) = 65.440 + 10.868X1 + 1.835X2-0.502X3-4.647X12 + 0.121X1X2-0.415X1X3-0.856X22 + 0.487X?2X3-1.789X32. The effects of NaCl concentration and pH on the WHC of the salt-soluble protein gel were extremely significant; but the low-temperature heating time was not. Response surface and contour were graphed with the WHC as the response value. The optimal extraction and gel preparation conditions of salt-soluble proteins from S. zygaena muscle were 0.90mol/L NaCl, pH7.0 and 35min of low-temperature heating time. Experimentally, these conditions produced a salt-soluble protein gel with a WHC of 72%, confirming the model prediction. |
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| Keywords: | Sphyrna zygaena Salt-soluble protein Water holding capacity (WHC) of gel Respond surface methodology |
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