| 海绵共附生微生物基因多态性的RAPD-PCR分析 |
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| 引用本文： | 李志勇,秦恩昊,何丽明,黄艳琴.海绵共附生微生物基因多态性的RAPD-PCR分析[J].海洋科学,2006,30(7):15-20. |
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| 作者姓名： | 李志勇 秦恩昊 何丽明 黄艳琴 |
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| 作者单位： | 上海交通大学,生命科学技术学院,海洋生物技术实验室,上海200240 |
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| 基金项目： | 国家863计划资助项目(2002AA628080,2004AA628060)，上海市科技启明星资助项目(04QMX1411) |
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| 摘 要： | 采用传统分离手段对中国南海海域的细薄星芒海绵（Stelletta tenui（Lindgren，1897）1、皱皮软海绵（Halichondria rugosa（Ridley＆Dendy））、澳大利亚厚皮海绵（Craniella australiensis（Carter））、贪婪倔海绵（Dysidea avara（Schmidt））4种海绵体内的微生物进行了分离培养，随机挑选64株芽孢杆菌属细菌（每种海绵16株）进行了RAPD-PCR基因多态性分析。研究表明，一些海绵微生物是可以通过传统分离手段得到的，来自同一或者不同海绵的微生物均具有丰富的基因多态性。
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| 关 键 词： | 海绵 芽孢杆菌 RAPD-PCR 聚类分析 |
| 文章编号： | 1000-3096（2006）07-0015-06 |
| 收稿时间： | 2003-10-10 |
| 修稿时间： | 2004-02-10 |
RAPD-PCR study on the genetic diversity of sponge-associated microorganisms |
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| LI Zhi-yong,QIN En-hao,HE Li-ming,HUANG Yan-qin.RAPD-PCR study on the genetic diversity of sponge-associated microorganisms[J].Marine Sciences,2006,30(7):15-20. |
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| Authors: | LI Zhi-yong QIN En-hao HE Li-ming HUANG Yan-qin |
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| Institution: | Marine Biotechnology Laboratory, School of Life Science and Biotechnology Shanghai Jiao Tong University, Shanghai 200240,China |
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| Abstract: | Sponge-associated microorganisms were isolated from 4 species of sponges Stelletta tenui (Lindgren, 1897), Halichondria rugosa (Ridley & Dendy), Craniella australiensis (Carter), Dysidea avara (Schmidt) in the South China Sea. After cultivation the genetic diversity of 64 strains of sponge-associated Bacillus (16 strains /sponge) was revealed based on RAPD-PCR fingerprinting and clustering analysis. It was proved that we can obtain sponge-associated microorganisms by traditional isolation method and there is genetic diversity among strains from the same or different sponges. |
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| Keywords: | sponge Bacillus RAPD-PCR clustering analysis |
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