Total oxyradical scavenging capacity of the deep-sea amphipod Eurythenes gryllus |
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| Authors: | L Camus B Gulliksen |
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| Institution: | a The University Centre on Svalbard, PB 156, 9170, Longyearbyen, Norway;b Akvamiljø as, Mekjarvik 12, 4070, Randaberg, Norway |
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| Abstract: | Environmental concern for the deep-sea ecosystem is increasing as contaminants, originating from anthropogenic activities, have been detected in deep-sea biota. However, little is known on the xenobiotics metabolising capability of deep-sea fauna. In this study, the deep-sea amphipod Eurythenes gryllus was selected as sentinel species to measure the total oxyradical scavenging capacity (TOSC). Individuals of E. gryllus were sampled at 2000 m depth in the Arctic Ocean. The TOSC assay was measured on the cytosolic fraction and the soluble fraction (3 kDa) of the digestive gland and on the cell-free haemolymph toward peroxyl, hydroxyl and peroxynitrite radicals according to the method of Winston et al. Free Radical Biology and Medicine 24 (3) (1998) 480] and Regoli and Winston Toxicology and Applied Pharmacology 156 (1999) 96]. These results provide the first baseline data set for total antioxidant capacity in a deep-sea amphipod. |
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