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| 南极生境真菌Gliocladium catenulatum T31菌株杀虫活性的研究 | |
| 引用本文： | 牛德庆,田黎,周俊英,郑立,陈靠山.南极生境真菌Gliocladium catenulatum T31菌株杀虫活性的研究[J].极地研究,2007,19(2):131-138. |
| 作者姓名： | 牛德庆 田黎 周俊英 郑立 陈靠山 |
| 作者单位： | 1. 国家海洋局第一海洋研究所现代化学分析室,青岛,266061 2. 国家海洋局第一海洋研究所现代化学分析室,青岛,266061;青岛科技大学生物系,青岛,266042 3. 青岛城阳区农业局,青岛,266109 4. 山东大学生命学院,济南,250100 |
| 基金项目： | 国家“863计划”(2007AA09Z435)，国家自然科学基金(20602009)资助 |
| 摘 要： | 本文对影响南极生境真菌Gliocladium catenulatum T31生长和杀虫活性的初始pH值、发酵时间、培养温度等影响因子进行了研究。并以小菜蛾为试虫,以校正死亡率为评价指标,利用微型平板法对真菌T31的杀虫活性进行测试,发酵液杀虫活性最高为60.42%。发酵物乙酸乙酯提取后,丙酮溶解0.33mg/mL浓度下,发酵液提取物杀虫活性最高为82.05%,菌体提取物杀虫活性为76.92%。盆栽试验证明该菌株发酵液对小菜蛾第7d的虫口减退率为54.2%。 |
| 关 键 词： | 南极真菌 杀虫活性 小菜蛾 盆栽试验 |
| 收稿时间： | 2007-02 |
| 修稿时间： | 2007-022007-05 |
A STUDY ON THE INSECTICIDAL ACTIVITY OF ANTARCTIC EPIPHYTE GLIOCLADIUM CATENULATUM T31 |
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| Niu Deqing,Tian li,Zhou Junying,Zhen li,Chen Kaoshan.A STUDY ON THE INSECTICIDAL ACTIVITY OF ANTARCTIC EPIPHYTE GLIOCLADIUM CATENULATUM T31[J].Chinese Journal of Polar Research,2007,19(2):131-138. | |
| Authors: | Niu Deqing Tian li Zhou Junying Zhen li Chen Kaoshan |
| Abstract: | In this study,the effects of major environmental factors on the growth and insecticidal activity of antarctic fungi Gliocladium catenulatum T31 were investigated.The method of micro-plate was employed.The bioactivity of the strain T31 to Diamondback Moth(Plutella xylostella) was studied with insect mortality as evaluated index.The most insecticidal activity of T31 fermentation broth was 60.42%.After acetic ether extracted T31 fermentation broth repeatedly,dissolved by acetone at the concentration of 0.33mg/mL,the best insecticidal activity of T31 fermentation broth extracts was 82.05% and insecticidal activity of T31 mycelium extracts was 76.92%.The potted experimentation showed that the controlling effect of T31 fermentation broth to Diamondback Moth was 54.2% on the seventh day. |
| Keywords: | Antarctic fungi insecticidal activity Plutella xylostella |
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